The protease-protected 30 kDa domain of SecA is largely inaccessible to the membrane lipid phase

The EMBO Journal
J EichlerW Wickner

Abstract

SecA binds to the inner membrane of Escherichia coli through low affinity lipid interactions or with high affinity at SecYEG, the integral domain of preprotein translocase. Upon addition of preprotein and nucleotide, a 30 kDa domain of SecYEG-bound SecA is protected from proteolysis via membrane insertion. Such protection could result from some combination of insertion into the lipid phase, into a proteinaceous environment or across the membrane. To assess the exposure of SecYEG-bound SecA to membrane lipids, a radiolabeled, photoactivatable and lipid-partitioning crosslinker, 3-trifluoromethyl-3-(m[125I]iodophenyl) diazirine benzoic acid ester, was incorporated into inner membrane vesicles. The 30 kDa domain of SecYEG-bound SecA, inserted into the membrane in response to translocation ligands, is 18-fold less labeled than SecY, which is labeled effectively. In contrast, incorporation of the purified 30 kDa SecA fragment into crosslinker-containing detergent micelles or addition of detergent to crosslinker-containing membranes bearing the protease-protected SecA domain readily allows for labeling of this domain. We propose that the protease-inaccessible 30 kDa SecA domain is shielded from the fatty acyl membrane phase by membra...Continue Reading

References

Jan 1, 1992·Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme·H Tokuda
Jan 1, 1991·Annual Review of Biochemistry·W WicknerF U Hartl
Jan 1, 1989·Methods in Enzymology·J Brunner
Jul 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·C A Kumamoto
Dec 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J B WeissP J Bassford
Aug 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·E Crooke, W Wickner
Jul 28, 1971·Journal of Molecular Biology·M S Bretscher
Nov 2, 1983·European Journal of Biochemistry·U HenningH Schaller
Aug 25, 1995·The Journal of Biological Chemistry·K DouvilleW Wickner
Feb 1, 1995·Trends in Biochemical Sciences·L L Randall, S J Hardy
Dec 9, 1994·Biochimica Et Biophysica Acta·R A Arkowitz, M Bassilana
Jan 1, 1993·Annual Review of Biochemistry·J Brunner
Mar 15, 1996·Science·G Schatz, B Dobberstein
Dec 6, 1996·The Journal of Biological Chemistry·A PriceW Wickner
Apr 1, 1996·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·K Ito
Apr 1, 1996·Trends in Cell Biology·J Brunner

❮ Previous
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Citations

Apr 6, 2004·Current Opinion in Microbiology·Hitoshi NakatogawaKoreaki Ito
Mar 14, 2000·FEMS Microbiology Letters·H KobayashiF Kawamura
Oct 13, 2001·Trends in Microbiology·H Mori, K Ito
Jan 5, 2000·Microbes and Infection·M G Schmidt, K B Kiser
Mar 6, 1999·Current Opinion in Microbiology·A J DriessenJ P van der Wolk
Apr 19, 2000·The Biochemical Journal·F Van Voorst, B De Kruijff
Aug 10, 2000·Molecular Microbiology·E H Manting, A J Driessen
Aug 16, 2000·The EMBO Journal·T L Yahr, W T Wickner
Mar 4, 2000·The EMBO Journal·E H MantingA J Driessen
Mar 14, 2012·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Jelger A Lycklama A Nijeholt, Arnold J M Driessen
Feb 17, 2005·Genes & Development·Hitoshi NakatogawaKoreaki Ito
Sep 7, 2000·Microbiology and Molecular Biology Reviews : MMBR·H TjalsmaJ M van Dijl
Feb 3, 1999·Annual Review of Genetics·P N Danese, T J Silhavy
May 27, 1997·Proceedings of the National Academy of Sciences of the United States of America·J Eichler, W Wickner
Mar 19, 2003·Proceedings of the National Academy of Sciences of the United States of America·Hong-Wei WangSen-Fang Sui
Dec 11, 1997·Cell·F DuongW Wickner
Nov 22, 2012·Journal of the American Chemical Society·Dorothy M KimJohn F Hunt
Sep 3, 2008·Molecular Microbiology·Spyridoula KaramanouAnastassios Economou
Nov 18, 2004·Biochimica Et Biophysica Acta·Eleftheria Vrontou, Anastassios Economou
Oct 31, 2002·The Journal of Biological Chemistry·Jordi BenachJohn F Hunt
Oct 22, 1998·FEBS Letters·B ShiltonA Economou
Oct 17, 2018·Journal of Bacteriology·Donald Oliver
Oct 6, 2007·Biopolymers·Eugenia M CléricoLila M Gierasch
May 21, 2003·Molecular Genetics and Genomics : MGG·N ShimokawaK Ito
Jul 11, 2001·The Journal of Biological Chemistry·A K VeenendaalA J Driessen
Aug 15, 1998·The Journal of Biological Chemistry·J EichlerW Wickner
Jan 25, 2000·The Journal of Biological Chemistry·C van der DoesA J Driessen
Jun 3, 2000·The Journal of Biological Chemistry·V Dapic, D Oliver
Oct 25, 2002·The Journal of Biological Chemistry·Yi-Te ChouLila M Gierasch
Apr 1, 2000·The Journal of Biological Chemistry·P PalestiniM Masserini
Oct 29, 1998·Journal of Bacteriology·J Eichler, W Wickner
Aug 4, 1999·Trends in Microbiology·A Economou
Nov 18, 2004·Biochimica Et Biophysica Acta·Annemieke van Dalen, Ben de Kruijff

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