The Protease Ste24 Clears Clogged Translocons

Cell
Tslil AstMaya Schuldiner

Abstract

Translocation into the endoplasmic reticulum (ER) is the first step in the biogenesis of thousands of eukaryotic endomembrane proteins. Although functional ER translocation has been avidly studied, little is known about the quality control mechanisms that resolve faulty translocational states. One such faulty state is translocon clogging, in which the substrate fails to properly translocate and obstructs the translocon pore. To shed light on the machinery required to resolve clogging, we carried out a systematic screen in Saccharomyces cerevisiae that highlighted a role for the ER metalloprotease Ste24. We could demonstrate that Ste24 approaches the translocon upon clogging, and it interacts with and generates cleavage fragments of the clogged protein. Importantly, these functions are conserved in the human homolog, ZMPSTE24, although disease-associated mutant forms of ZMPSTE24 fail to clear the translocon. These results shed light on a new and critical task of Ste24, which safeguards the essential process of translocation.

References

Jul 1, 1996·The Journal of Cell Biology·D T NgP Walter
Sep 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·W K SchmidtS Michaelis
Jun 1, 2000·The Journal of Cell Biology·T SuzukiW J Lennarz
Jan 5, 2002·Molecular and Cellular Biology·Efrat RabinovichShoshana Bar-Nun
Apr 16, 2002·Molecular Biology of the Cell·Donald J Tipper, Carol A Harley
Jul 26, 2002·Nature·Guri GiaeverMark Johnston
Dec 9, 2003·Nature·Bert Van den BergTom A Rapoport
Aug 4, 2005·The Journal of Biological Chemistry·Machiko SakohYoshinori Akiyama
Feb 17, 2006·Journal of Biochemical and Biophysical Methods·Fusinita van den Ent, Jan Löwe
Jul 19, 2006·Proceedings of the National Academy of Sciences of the United States of America·Hyun KimGunnar von Heijne
Aug 19, 2006·Proceedings of the National Academy of Sciences of the United States of America·Archana BelleErin K O'Shea
Nov 4, 2006·Yeast·Brian K Sato, Randolph Y Hampton
Jul 27, 2007·Proceedings of the National Academy of Sciences of the United States of America·Catherine CoffinierLoren G Fong
Jul 16, 2008·Nature Methods·David K BreslowJonathan S Weissman
Jun 30, 2009·Biological Chemistry·Przemyslaw GrudnikIrmgard Sinning
Jan 23, 2010·Science·Michael CostanzoCharles Boone
May 1, 2010·The Journal of Biological Chemistry·Shruthi S VembarJeffrey L Brodsky
Sep 10, 2010·The Journal of Biological Chemistry·Yoichiro HaradaWilliam J Lennarz
Dec 27, 2011·Cell·Sichen Shao, Ramanujan S Hegde
Jan 10, 2012·Annual Review of Biophysics·Eunyong Park, Tom A Rapoport
Jun 13, 2012·The Journal of Cell Biology·Eric M RubensteinMark Hochstrasser
Jun 22, 2012·Human Molecular Genetics·Jemima BarrowmanSusan Michaelis
Aug 31, 2012·Microbiology and Molecular Biology Reviews : MMBR·Susan Michaelis, Jemima Barrowman
Mar 28, 2013·Critical Reviews in Biochemistry and Molecular Biology·Tslil Ast, Maya Schuldiner
Mar 30, 2013·Science·Susan Michaelis, Christine A Hrycyna
Mar 30, 2013·Science·Edward E PryorMark E Dumont
Mar 30, 2013·Science·Andrew QuigleyElisabeth P Carpenter
Dec 7, 2013·BMC Bioinformatics·Barry P Young, Christopher J R Loewen
Mar 26, 2014·Current Opinion in Cell Biology·Naama Aviram, Maya Schuldiner
May 23, 2014·Journal of Cell Science·Tslil AstMaya Schuldiner

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Citations

Mar 24, 2016·Traffic·Giorgia Brambilla Pisoni, Maurizio Molinari
Aug 20, 2016·Scientific Reports·Reiko KintakaHisao Moriya
Oct 25, 2016·Protein Science : a Publication of the Protein Society·Kathleen M ClarkMark E Dumont
Jan 26, 2017·Analytical Biochemistry·Buenafe T Arachea, Michael C Wiener
Apr 2, 2016·Journal of Cell Science·Yuexia WangHoward J Worman
Aug 8, 2018·Acta Crystallographica. Section D, Structural Biology·Brandon R GoblirschMichael C Wiener
Nov 3, 2016·F1000Research·Sita Reddy, Lucio Comai
Apr 25, 2019·PloS One·Francesco EliaKarin Römisch
Apr 14, 2016·The Journal of Cell Biology·Caitlin Sedwick
Jul 14, 2019·Experimental Biology and Medicine·Cecilia ÖstlundHoward J Worman
Jan 24, 2019·Cold Spring Harbor Perspectives in Biology·Patrick G NeedhamJeffrey L Brodsky
Aug 17, 2019·Journal of Cell Science·Nathalie KühnleMarius K Lemberg
May 1, 2016·The Journal of Biological Chemistry·Emily R HildebrandtWalter K Schmidt
Oct 18, 2019·JCI Insight·Daniel BraysonCatherine M Shanahan
Jul 11, 2019·The Journal of Cell Biology·Yury S BykovJohn A G Briggs
Apr 30, 2020·The Journal of Cell Biology·Nurulain HoGuillaume Thibault
Mar 2, 2017·The Journal of Experimental Medicine·Bishi FuMartin E Dorf
Apr 11, 2019·Frontiers in Molecular Biosciences·Sigal Rencus-LazarDana Laor
Nov 23, 2017·Frontiers in Physiology·Sven LangRichard Zimmermann
May 24, 2019·Nature·Christoph U MårtenssonThomas Becker
May 30, 2019·Nature·Sylvie Callegari, Peter Rehling
Jun 20, 2020·The Journal of Cell Biology·Danming TangShahram Misaghi
May 11, 2016·Human Molecular Genetics·Gali MaorMia Horowitz
Oct 10, 2020·The Journal of Biological Chemistry·Avery M RunnebohmEric M Rubenstein
Dec 10, 2020·The Journal of Biological Chemistry·Timothy D BabatzSusan Michaelis
Jan 26, 2021·Frontiers in Cell and Developmental Biology·Lihui Wang, Yihong Ye
May 15, 2020·Current Opinion in Cell Biology·Ben P PhillipsElizabeth A Miller
Apr 16, 2021·Current Opinion in Structural Biology·Ben P Phillips, Elizabeth A Miller
Nov 2, 2020·Biochimica Et Biophysica Acta. Proteins and Proteomics·Marcel V Alavi
Jan 10, 2019·Methods : a Companion to Methods in Enzymology·Eric D SpearSusan Michaelis
Oct 15, 2021·Nature Reviews. Immunology·Saliha Majdoul, Alex A Compton

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