The proteasomal ATPase complex is required for stress-induced transcription in yeast

Nucleic Acids Research
Rita SulahianThomas Kodadek

Abstract

Sug1 and Sug2 are two of six ATPases in the 19S regulatory particle of the 26S proteasome. We have shown previously that these proteins play a non-proteolytic role in the transcription of the GAL genes in yeast. In this study, we probe the requirement for these factors in stress-induced transcription in yeast. It is known that proteasomal proteolysis is not required for these events. Indeed, proteasome inhibitors strongly stimulate expression of these stress response genes. However, shifting strains carrying temperature-sensitive alleles of SUG1 and SUG2 to the restrictive temperature strongly inhibited the expression of HSP26, HSP104 and GAD1 in response to heat shock or treatment with menadione bisulfate. Furthermore, chromatin immunoprecipitation analysis revealed the recruitment of Sug1, Sug2 and Cim5 (another of the ATPases), but not 20S proteasome core proteins, to the promoters of these genes. These data show that the non-proteolytic requirement for the proteasomal ATPases extends beyond the GAL genes in yeast and includes at least the heat and oxidative stress-responsive genes.

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Citations

Dec 15, 2006·Genes & Development·Anwarul FerdousStephen Albert Johnston
Nov 21, 2007·Biochemistry. Biokhimii︠a︡·N N GesslerT A Belozerskaya
Oct 3, 2012·Genetics·Daniel FinleyPeter Kaiser
Oct 18, 2006·Proceedings of the National Academy of Sciences of the United States of America·Sumit AgarwalNikunj V Somia
Dec 12, 2007·Nature Reviews. Genetics·Suzanne Komili, Pamela A Silver
May 28, 2008·The Journal of Cell Biology·Damien LaporteIsabelle Sagot
Dec 6, 2008·Critical Reviews in Biochemistry and Molecular Biology·Sukesh R Bhaumik, Shivani Malik
Aug 31, 2010·Biochimica Et Biophysica Acta·Sukesh R Bhaumik
Oct 27, 2009·Journal of Molecular Biology·Agnieszka D TruaxSusanna F Greer
Aug 8, 2009·Biochimica Et Biophysica Acta·Olivia I KouesSusanna F Greer
Oct 25, 2008·Yeast·Keren Baranes-BacharDina Raveh
Dec 20, 2011·Biochimica Et Biophysica Acta·Encar García-OliverSusana Rodríguez-Navarro
Oct 6, 2007·The Journal of Biological Chemistry·Peng Yu, Thomas Kodadek
Jul 14, 2006·The Journal of Biological Chemistry·Devanjan SikderThomas Kodadek
Feb 3, 2021·Molecular and Cellular Biology·Jason J Kwon, William C Hahn
Jan 25, 2007·ACS Chemical Biology·Anna K Mapp, Aseem Z Ansari
Mar 7, 2009·Chemical Reviews·António J MarquesR Jürgen Dohmen
Aug 21, 2010·Biochimica Et Biophysica Acta·Jaechan KwakDaeyoup Lee

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Methods Mentioned

BETA
immunoprecipitation
ChIP
PCR

Software Mentioned

Excel

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