PMID: 8612623Mar 1, 1996Paper

The proteasome subunit, C2, contains an important site for binding of the PA28 (11S) activator

European Journal of Biochemistry
M A KaniaA L Goldberg

Abstract

The PA28, or 11S regulatory complex, stimulates the peptidase activities of the 20S proteasome. Monoclonal antibodies were screened for their ability to inhibit the activation by PA28 of proteasomes from rabbit reticulocytes. We identified one antibody that inhibited proteasome activation by PA28 and dissociated formed proteasome-PA28 complexes. A fourfold molar excess of antibody to proteasome markedly reduced the PA28 activation of three peptidase activities. Examination of proteasome-antibody mixtures by electron microscopy revealed that the antibody formed chains of proteasomes, and digital image analysis of individual proteasomes demonstrated that the antibody binds to the outer alpha rings. This antibody recognizes proteasome subunit C2, which we conclude contains an important contact site for the PA28 activator. However, the antibody did not block proteasome activation by PA700, or 19S regulator, which also associates with the alpha rings. Thus, these two regulators appear to bind to the proteasome at different sites.

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Citations

Sep 11, 2002·Burns : Journal of the International Society for Burn Injuries·Jiake ChaiZhiyong Sheng
Apr 17, 2002·The Journal of Experimental Medicine·Ulrike KuckelkornUlrich Steinhoff
May 29, 2002·The EMBO Journal·Paolo CascioAlfred L Goldberg
Jun 10, 2003·Critical Care Medicine·Jiake ChaiZhiyong Zy Sheng
Jun 8, 1999·Annual Review of Immunology·K L Rock, A L Goldberg
Jan 23, 2008·Biological Chemistry·Nicolle Breusing, Tilman Grune
Jul 1, 1996·Biological Chemistry·A WehrenB Dahlmann
Dec 14, 2004·FEBS Letters·Deborah A Ferrington, Rebecca J Kapphahn
Jun 16, 2015·Free Radical Biology & Medicine·Betul KarademirNesrin Kartal Ozer
Apr 18, 2009·Molecular Aspects of Medicine·Tobias JungTilman Grune
Mar 28, 1997·The Journal of Biological Chemistry·M SeegerW Dubiel
Jan 29, 2005·Chembiochem : a European Journal of Chemical Biology·Michael GrollRobert Huber
Jan 4, 2001·Nature Genetics·X XuG P Nolan
Oct 12, 2002·Molecular & Cellular Proteomics : MCP·Stephane ClaverolBernard Monsarrat
Mar 9, 1999·Archives of Biochemistry and Biophysics·B DahlmannK B Hendil

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