PMID: 3383850Jun 1, 1988Paper

The protection by ascorbate and glutathione against microsomal lipid peroxidation is dependent on vitamin E

European Journal of Biochemistry
H Wefers, H Sies

Abstract

Lipid peroxidation of rat liver microsomal fractions was monitored by its low-level chemiluminescence in preparations from controls and vitamin-E-deficient animals. Measurements were made (a) of the duration of the lag phase tau0 after initiation with NADPH/iron-ADP and (b) of the slope of the chemiluminescence increase. In microsomes with normal vitamin E (alpha-tocopherol) level the lag phase tau0 was substantially increased by ascorbate; in contrast, even an enhanced peroxidation was observed with ascorbate in vitamin-E-deficient microsomes. Therefore, the ascorbate-mediated protection of microsomal membranes against lipid peroxidation is dependent on vitamin E in the membrane. In vitamin E deficiency the pro-oxidant effect of ascorbate was abolished when glutathione (GSH) was present. Likewise, GSH does not prolong the lag phase tau0 in vitamin E deficiency. However, GSH (but not cysteine) exerts an antioxidant effect both in controls and in vitamin E deficiency by decreasing the slope of the chemiluminescence increase during lipid peroxidation. The involvement of GSH in an enzyme-dependent mechanism is suggested.

References

Nov 15, 1985·Experientia·P LambeletJ Löliger
Jan 1, 1987·Annals of the New York Academy of Sciences·E Niki
Aug 1, 1986·Journal of Applied Physiology·D P Franco, S G Jenkinson
Mar 29, 1985·Biochimica Et Biophysica Acta·F UrsiniC Gregolin
Feb 15, 1983·Archives of Biochemistry and Biophysics·G W BurtonK U Ingold

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Citations

Apr 1, 1992·Journal of Bioluminescence and Chemiluminescence·C Pascual, C Romay
May 1, 1991·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·E EndreffyK Kása
Jan 1, 1997·Journal of Neural Transmission·R K PearceC D Marsden
Sep 8, 1993·Molecular and Cellular Biochemistry·M MukhopadhyayI B Chatterjee
Sep 1, 1995·Biulleten' eksperimental'noĭ biologii i meditsiny·L F DmitrievA V Lebedev
Jul 1, 1996·Lipids·A Kamal-Eldin, L A Appelqvist
Jan 1, 1995·Biological Trace Element Research·E Brezezińska-SlebodzińskaG Wieczorek
Sep 1, 2006·Indian Journal of Clinical Biochemistry : IJCB·M Maneesh, H Jayalekshmi
Mar 1, 2006·Indian Journal of Clinical Biochemistry : IJCB·S MohantyA Panda
Jun 6, 2007·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·David CostantiniGiacomo Dell'Omo
Nov 3, 1992·Biochemical Pharmacology·A I CederbaumH Speisky
Jan 1, 1989·The International Journal of Biochemistry·E BaysalA Stern
Aug 8, 1995·Mechanisms of Ageing and Development·K Y TuK S Matthews
Jan 1, 1990·Pharmacology & Therapeutics·X Q ShanD P Jones
Jan 1, 1994·Pharmacology & Therapeutics·M E GötzM B Youdim
Jul 1, 1991·Archives of Gerontology and Geriatrics·J FerrerJ Miquel
Dec 1, 1992·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·A Hudecová, E Ginter
Jan 1, 1990·Free Radical Biology & Medicine·C J Doelman, A Bast
Jan 1, 1991·Free Radical Biology & Medicine·C K Chow
Mar 1, 1994·Free Radical Biology & Medicine·S PinamontiA Ciaccia
Apr 1, 1994·Free Radical Biology & Medicine·S ChakrabortyI B Chatterjee
Jan 1, 1991·Journal of Photochemistry and Photobiology. B, Biology·H Sies, M E Murphy
Dec 23, 2003·Clinica Chimica Acta; International Journal of Clinical Chemistry·Ntei AbuduStanley S Levinson
Feb 18, 2004·Clinica Chimica Acta; International Journal of Clinical Chemistry·Suleyman AydinFrazilet Erman
Aug 29, 2013·Environmental Toxicology and Pharmacology·Bandari Uma MaheshRamakrishna Sistla
May 17, 2003·Fertility and Sterility·Ashok AgarwalMohamed A Bedaiwy
Oct 18, 2002·Neuropharmacology·E Rodríguez-MartínM A Mena
May 14, 1998·Molecular Aspects of Medicine·A TaylorM Obin

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