The protective effect of epicatechin on experimental ulcerative colitis in mice is mediated by increasing antioxidation and by the inhibition of NF-κB pathway

Pharmacological Reports : PR
HaiJing ZhangWenJie Wang

Abstract

Ulcerative colitis (UC) is a chronic inflammatory intestinal disease. It is necessary to find out new effective drugs for UC. In our study epicatechin extracted from grape seed by our institute for the first time could treat UC effectively. Then anti-UC mechanisms of epicatechin were elucidated in vivo and in vitro. Dextran sulfate sodium (DSS)-induced acute UC mice model was used to evaluate the activity of epicatechin and its properties against UC. Then its anti-inflammatory and antioxidant effects were evaluated as follows: the concentrations of TNF-α and IL-6 in the colon supernatants were determined by ELISA. NO and MPO were assayed by Griess method and commercial kit respectively. NF-κB were determined by NF-κB-Dependent Reporter Gene Expression Assay and Western Blotting respectively. Antioxidant factors such as SOD, MDA, GSH-Px and CAT were also measured in colon tissues and cell supernatant stimulated by LPS respectively. In C57BL/6J mice model with DSS-induced UC, epicatechin was able to decrease the disease activity index and colon macroscopic damage index scores, reduce body weight loss, and significantly relieve colon contracture and crypt damage. TNF-α, IL-6, NO, MPO and MDA were reduced in the mice administered e...Continue Reading

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Citations

Mar 12, 2015·The American Journal of Pathology·Mark O DeNichiloAndreas Evdokiou
Sep 13, 2012·Advances in Medical Sciences·O Stasikowska-KanickaM Wągrowska-Danilewicz
Jul 25, 2017·Molecular Nutrition & Food Research·Hui ChengQinglin Li
Jul 16, 2019·Future Medicinal Chemistry·Shuang YangYinghong Wang
Feb 25, 2020·Critical Reviews in Food Science and Nutrition·Zhihao QuSheng Zhang
Feb 18, 2017·World Journal of Gastroenterology : WJG·Hong-Ying ZhaoYun-Feng Liu
Oct 2, 2019·Experimental and Therapeutic Medicine·Suxiao Wu, Zilan Chen
Jul 25, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Shikai SunJing Qu
Nov 23, 2021·Human & Experimental Toxicology·Reza AzadnasabMohammad Javad Khodayar

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