The Protective Effect of Fluorofenidone against Cyclosporine A-Induced Nephrotoxicity

Kidney & Blood Pressure Research
Yang ChenZhangzhe Peng

Abstract

Cyclosporine A (CsA) is an immunosuppressant drug that is used during organ transplants. However, its utility is limited by its nephrotoxic potential. This study aimed to investigate whether fluorofenidone (AKF-PD) could provide protection against CsA-induced nephrotoxicity. Eighty-five male Sprague-Dawley rats were divided into 5 groups: drug solvent, CsA, CsA with AKF-PD (250, 500 mg/kg/day), and CsA with pirfenidone (PFD, 250 mg/kg/day). Tubulointerstitial injury index, extracellular matrix (ECM) deposition, expression of type I and IV collagen, transforming growth factor (TGF)-β1, platelet-derived growth factor (PDGF), Fas ligand (FASL), cleaved-caspase-3, cleaved-poly(ADP-ribose) polymerase (PARP)-1, and the number of transferase-mediated nick end-labeling (TUNEL)-positive renal tubule cells were determined. In addition, levels of TGF-β1, FASL, cleaved-caspase-3, cleaved-PARP-1, and number of annexin V-positive cells were determined in rat proximal tubular epithelial cells (NRK-52E) treated with CsA (20 μmol/L), AKF-PD (400 μg/mL), PFD (400 μg/mL), and GW788388 (5 μmol/L). AKF-PD (250, 500 mg/kg/day) significantly reduced tubulointerstitial injury, ECM deposition, expression of type I and IV collagen, TGF-β1, PDGF, FASL, c...Continue Reading

References

Jan 1, 1986·Annual Review of Medicine·W M Bennett, D J Norman
Sep 13, 1984·The New England Journal of Medicine·B D MyersM Perlroth
Mar 10, 1995·Science·S Nagata, P Golstein
Jan 1, 1995·Annual Review of Physiology·H E Abboud
May 1, 1997·Kidney International·W A Border, N A Noble
Apr 29, 1998·Internal Medicine·S Nagata
Sep 5, 2001·Kidney International·L D TruongW N Suki
Apr 19, 2002·Nephron·Mohammed S RazzaqueTakashi Taguchi
Jun 11, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Naoki HagimotoNobuyuki Hara
Jun 23, 2004·Cytokine & Growth Factor Reviews·James C Bonner
Mar 15, 2006·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Wei CaoZeneng Cheng
Jun 26, 2007·Nature Reviews. Immunology·Peter H KrammerInna N Lavrik
Oct 31, 2007·Apoptosis : an International Journal on Programmed Cell Death·H ServaisM-P Mingeot-Leclercq
Feb 17, 2009·Clinical Journal of the American Society of Nephrology : CJASN·Maarten NaesensMinnie Sarwal
May 20, 2011·Molecular and Cellular Biochemistry·Bing-Xin LiLi-Jian Tao
Feb 27, 2013·European Journal of Clinical Investigation·Yu PengLijian Tao
Dec 18, 2013·American Journal of Physiology. Gastrointestinal and Liver Physiology·Yu PengLijian Tao

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Jan 7, 2021·International Journal of Molecular Sciences·Christopher L MooreAlexei G Basnakian
Feb 4, 2021·Diabetes, Metabolic Syndrome and Obesity : Targets and Therapy·Hengmei ZhuXiaojiang Zhan

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