The protective effect of ischemic preconditioning associated with altered gene expression profiles in intestinal grafts after reperfusion

The Journal of Surgical Research
Shufeng WangGuowei Li

Abstract

Ischemia-reperfusion injury after small bowel transplantation (SBT) has been considered as a trigger for early graft rejection. Ischemic preconditioning (IPC) may improve the tolerance of the small intestine to ischemic insults; however, the mechanism of IPC is still not completely elucidated. Accordingly, the aim of the present research is to investigate the molecular mechanism of IPC through a large-scale expression measurement of intragraft gene profiles by complementary DNA microarrays in SBT. Three groups of rats were studied: S group (sham group), SBT group, and ISBT group (IPC plus SBT), in which IPC was carried out before the graft was harvested. Samples were collected from the different groups 1 h after reperfusion. Gene expression profiles of intestinal graft from the SBT group or ISBT group relative to gene expression profiles of normal intestine from the S group were analyzed respectively using 4096 gene chips. Microarray analysis showed that 14 genes were up-regulated more than 2-fold after reperfusion compared with normal intestine, and 17 genes were down-regulated in the SBT group. In the ISBT group, 84 genes with differential expression were detected after reperfusion compared with normal intestine. It was found...Continue Reading

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Citations

Jul 1, 2015·Experimental and Molecular Pathology·Zuzana JonecovaPeter Kruzliak
Aug 19, 2014·Transplantation Proceedings·R A M CamprodonJ de Oca

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