The protective effect of ischemic preconditioning associated with altered gene expression profiles in rat lung after reperfusion

The Journal of Surgical Research
Nie JunWang Jianjun

Abstract

Ischemia-reperfusion (IR) injury remains an important clinical problem after lung transplantation. It is well-known that ischemic preconditioning (IPC) markedly enhances the ability of organs to withstand a sustained ischemia and reperfusion injury. But the molecular mechanisms of this protective effect are poorly understood. Therefore, we used gene microarrays to profile gene expression patterns involved in ischemia precondition. Three groups of rats were studied: IR group (IR 0, 1, 3, 6, 24, n = 5 each group, IPC group (IP 1, IP 3, 6, 24 h, n = 5 each group) and Sham group (n = 5). Samples were collected from different groups. The RatRef-12 expression Beadchip (22,226 gene probes per array) was used to analyze the pattern of gene expression in all groups. Microarray analysis showed that 648, 340, 711, 1279, and 641 genes were differentially expressed in IR 0-, 1-, 3-, 6- and 24-h groups, respectively. The profile revealed that IPC induced significant alterations of expression of many genes encoding inflammatory associated factors, oxidation and antioxidant molecules, apoptosis regulatory protein, metabolic enzyme, and ion channel protein. The damage and repair after lung IR are a dynamic process. IPC affects gene expression p...Continue Reading

References

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Citations

Mar 17, 2012·European Journal of Cardio-thoracic Surgery : Official Journal of the European Association for Cardio-thoracic Surgery·Carlos SimónFederico González-Aragoneses
Aug 11, 2012·PloS One·Vicki L MahanFrank A Pigula
Jun 20, 2013·American Journal of Respiratory and Critical Care Medicine·Anthony W CastleberryMatthew G Hartwig
Jun 9, 2015·Experimental Lung Research·Chou-Chin LanChin-Pyng Wu
Aug 2, 2013·Journal of the Formosan Medical Association = Taiwan Yi Zhi·Min Gao, Donglei Liu
Sep 20, 2018·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Peter DromparisBenjamin A Adam
Jul 10, 2013·Experimental and Therapeutic Medicine·Cimen Gülben OlgunerSerap Cilaker Micili

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