The protective effects of prostaglandin E1 on sinusoidal endothelial cells in xenogeneic pig liver perfusion

The Journal of Surgical Research
T YagiY Yamaoka

Abstract

The effects of prostaglandin E1 (PGE1) on hepatic sinusoidal endothelial cells (SEC) in the xenogeneic immunoreaction were investigated. Porcine livers were perfused with fresh human blood via the portal vein (PV) and the hepatic artery (HA) either with the administration of PGE1 (Group PG) or without PGE1 (Group C). The creatine kinase-BB component (CK-BB) in the perfusate was measured to assess SEC damage. SEC activation and complement activation were evaluated immunohistochemically by the expression of von Willebrand factor (vWF) and by the deposition of membrane attack complex (MAC), respectively. Xenoperfusion in Group C was discontinued between 4 and 6 hr due to the rapid elevation of HA pressures and the massive loss of perfusate. In Group PG, both PV and HA pressures were kept stable for up to 9 hr. In Group C, severe interlobular bleeding and diffuse extrasinusoidal hemorrhage were observed at 4 hr histologically, while in Group PG, the hepatic architecture was maintained without hemorrhage at 6 hr. MAC was markedly deposited on SEC and parenchymal cells at 3 hr in both groups. The amount of vWF, however, was expressed on SEC in large amounts at 1 hr in Group C, while small amounts were expressed at 1 hr in Group PG. I...Continue Reading

References

Nov 1, 1979·The Journal of Clinical Investigation·S L KunkelR B Zurier
Mar 1, 1990·The Biochemical Journal·B SmedsrødT C Laurent
Oct 1, 1990·Transplantation·J S Pober, R S Cotran
Jun 1, 1989·Transplantation Proceedings·R J Duquesnoy
Jul 1, 1985·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·E WisseR S McCuskey
Apr 1, 1981·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·S M HsuH Fanger
Mar 1, 1983·Experimental Cell Research·S ErikssonB Smedsrød
Jan 1, 1994·The Journal of Experimental Medicine·A F CoughlanW W Hancock
Oct 1, 1994·Immunological Reviews·J L Platt
Oct 1, 1994·Immunological Reviews·F H BachW W Hancock
Sep 1, 1994·The Journal of Surgical Research·K HanazakiF Iida
Apr 1, 1994·The Journal of Surgical Research·T S HellingC L Eisenstein
Mar 1, 1993·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·M VaubourdolleJ Giboudeau

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