The protein arginine methyltransferases CARM1 and PRMT1 cooperate in gene regulation.

Nucleic Acids Research
Markus A KleinschmidtUta-Maria Bauer

Abstract

Protein arginine methyltransferases (PRMT) have been implicated in the regulation of transcription. They are recruited to promoters via interaction with transcription factors and exert their coactivator function by methylating arginine residues in histones and other chromatin proteins. Here, we employ an unbiased approach to identify novel target genes, which are under the control of two members of the enzyme family, PRMT1 and CARM1/PRMT4 (coactivator associated arginine methyltransferase 1). By using cDNA microarray analysis, we find that the siRNA-mediated single knockdown of neither CARM1 nor PRMT1 causes significant changes in gene expression. In contrast, double knockdown of both enzymes results in the deregulated expression of a large group of genes, among them the CITED2 gene. Cytokine-stimulated expression analysis indicates that transcriptional activation of CITED2 depends on STAT5 and the coactivation of both PRMTs. ChIP analysis identifies the CITED2 gene as a direct target gene of STAT5, CARM1 and PRMT1. In reporter gene assays, we show that STAT5-mediated transcription is cooperatively enhanced by CARM1 and PRMT1. Interaction assays reveal a cytokine-induced association of STAT5 and the two PRMTs. Our data demonstr...Continue Reading

References

Nov 13, 1998·Proceedings of the National Academy of Sciences of the United States of America·H B SunY C Yang
Nov 13, 1998·The Journal of Biological Chemistry·A LischkeK Friedrich
Jun 26, 1999·Science·D ChenM R Stallcup
Sep 30, 2000·The Journal of Biological Chemistry·D ChenM R Stallcup
Apr 20, 2001·Proceedings of the National Academy of Sciences of the United States of America·V G TusherG Chu
Dec 26, 2001·EMBO Reports·Uta-Maria BauerTony Kouzarides
Sep 5, 2002·Nature Reviews. Molecular Cell Biology·David E Levy, J E Darnell
Oct 11, 2002·The EMBO Journal·Martine Chevillard-BrietLaurence Vandel
Dec 25, 2002·Current Biology : CB·Sylvain DaujatTony Kouzarides
Apr 22, 2003·Genes & Development·Natalie Rezai-ZadehEdward Seto
May 22, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Weiguo ChenGurjit K Khurana Hershey
Aug 26, 2004·Molecular Cell·Kerri A MowenLaurie H Glimcher
Sep 2, 2004·Cell·Graeme L CuthbertTony Kouzarides
Nov 20, 2004·Cell·Torsten MeissnerUwe Vinkemeier
Dec 15, 2004·Journal of Virology·Marie-Chloé BoulangerStéphane Richard
Feb 17, 2005·Science's STKE : Signal Transduction Knowledge Environment·François-Michel BoisvertStéphane Richard
Feb 26, 2005·Proceedings of the National Academy of Sciences of the United States of America·Young-Ho LeeMichael R Stallcup
Apr 14, 2005·The Journal of Biological Chemistry·Waraporn KomyodIris Behrmann
May 4, 2005·Molecular Cell·Mark T Bedford, Stéphane Richard
Jun 21, 2005·Genes & Development·Catherine TeyssierMichael R Stallcup
Dec 27, 2005·Current Drug Targets. Immune, Endocrine and Metabolic Disorders·Ilka Wittig, Bernd Groner
Feb 21, 2006·Biochemical and Biophysical Research Communications·Jeffry R CookSidney Pestka
Mar 2, 2006·Molecular and Cellular Biology·Tatsuji FujiwaraMasaya Tohyama

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Citations

Nov 10, 2009·The Journal of Biological Chemistry·Daehoon KimMark T Bedford
Mar 13, 2009·The Journal of Biological Chemistry·Markus A KleinschmidtStefan Gaubatz
Jan 13, 2009·Genes & Development·Susanne WeberUta-Maria Bauer
Jun 21, 2011·Eukaryotic Cell·John C Fisk, Laurie K Read
Nov 26, 2010·The Journal of Clinical Investigation·Patrick G GallagherDavid M Bodine
Mar 19, 2013·PLoS Genetics·Gundula StreubelUta-Maria Bauer
Nov 16, 2010·FEBS Letters·Alessandra Di Lorenzo, Mark T Bedford
Nov 7, 2013·Autoimmunity·Hester A DoyleMark J Mamula
Apr 5, 2016·Mediators of Inflammation·Ji Hye KimJae Youl Cho
Nov 26, 2009·DNA Repair·M StefaniniD Orioli
Aug 1, 2009·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Thomas B NicholsonStéphane Richard
Sep 9, 2008·Neuroscience Letters·Shingo MiyataMasaya Tohyama
Sep 24, 2015·Biochimica Et Biophysica Acta·Dariusz ZakrzewiczMalgorzata Wygrecka
Sep 30, 2014·Advances in Biological Regulation·Sanzida Jahan, James R Davie
Sep 24, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Weizheng ZhouYang Yuan
Mar 21, 2012·The Biochemical Journal·Shu-Ching Mary WangGeorge E O Muscat
Dec 22, 2010·Molecular Cell·Yanzhong YangMark T Bedford
Jan 20, 2009·Molecular Cell·Mark T Bedford, Steven G Clarke
Nov 2, 2017·Antioxidants & Redox Signaling·Mei-Ling YangMark J Mamula
Nov 12, 2009·British Journal of Cancer·T van AgthovenL C J Dorssers
Mar 20, 2013·Current Opinion in Hematology·Jinwei Du, Yu-Chung Yang
Feb 23, 2017·Frontiers in Immunology·Paulo C RodriguezAmir A Al-Khami
Apr 26, 2015·The Journal of Biological Chemistry·Yalemi MoralesJoan M Hevel
Feb 9, 2018·Journal of Dental Research·T ZhangJ Xu
Aug 29, 2019·American Journal of Physiology. Lung Cellular and Molecular Physiology·Rim S J SarkerAli Önder Yildirim
Nov 21, 2018·Life Science Alliance·Donghang ChengMark T Bedford
Oct 4, 2020·The Journal of Biological Chemistry·Donghang ChengMark T Bedford

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Methods Mentioned

BETA
ChIP
immuoprecipitation
PCR
transfection
FCS
pulldown
pulldowns
coimmunoprecipitation
immunoprecipitation
nuclear translocation

Software Mentioned

R
MIAME
Array Express

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