The protein disulfide isomerase 1 of Phytophthora parasitica (PpPDI1) is associated with the haustoria-like structures and contributes to plant infection

Frontiers in Plant Science
Yuling MengWeixing Shan

Abstract

Protein disulfide isomerase (PDI) is a ubiquitous and multifunction enzyme belonging to the thioredoxin (TRX) superfamily, which can reduce, oxidize, and catalyze dithiol-disulfide exchange reactions. Other than performing housekeeping functions in helping to maintain proteins in a more stable conformation, there is some evidence to indicate that PDI is involved in pathogen infection processes. In a high-throughput screening for necrosis-inducing factors by Agrobacterium tumefaciens-mediated transient expression assay, a typical PDI gene from Phytophthora parasitica (PpPDI1) was identified and confirmed to induce strong cell death in Nicotiana benthamiana leaves. PpPDI1 is conserved in eukaryotes but predicted to be a secreted protein. Deletion mutant analyses showed that the first CGHC motif in the active domain of PpPDI1 is essential for inducing cell death. Using P. parasitica transformation method, the silencing efficiency was found to be very low, suggesting that PpPDI1 is essential for the pathogen. Translational fusion to the enhanced green fluorescent protein (EGFP) in stable P. parasitica transformants showed that PpPDI1 is associated with haustoria-like structures during pathogen infection. Furthermore, the PpPDI1-EGF...Continue Reading

References

Nov 1, 1991·Molecular Plant-microbe Interactions : MPMI·H S JudelsonR W Michelmore
May 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·M LaMantiaT Mizunaga
Sep 1, 1993·Molecular Plant-microbe Interactions : MPMI·S KamounB M Tyler
May 1, 1997·Molecular Plant-microbe Interactions : MPMI·M HahnK Mendgen
Dec 3, 1999·Microbes and Infection·J Yu, J S Kroll
Jun 8, 2001·Proceedings of the National Academy of Sciences of the United States of America·R T VoegeleK Mendgen
Jul 5, 2001·Proceedings of the National Academy of Sciences of the United States of America·L J Szabo, W R Bushnell
Oct 18, 2002·Journal of Cellular Physiology·Carlo TuranoAnna Ferraro
Nov 2, 2002·The Plant Journal : for Cell and Molecular Biology·Dinah QutobMark Gijzen
Nov 2, 2002·The Plant Journal : for Cell and Molecular Biology·Guido FellbrichThorsten Nürnberger
Apr 20, 2005·Proceedings of the National Academy of Sciences of the United States of America·Alexander LevitanAvihai Danon
Jun 30, 2006·International Journal for Parasitology·B MahajanS Kumar
Aug 15, 2006·Molecular Plant-microbe Interactions : MPMI·Thirumala-Devi KannegantiSophien Kamoun
Aug 25, 2006·Journal of Virology·Joanna GilbertThomas Benjamin
Mar 9, 2007·FEMS Microbiology Letters·Ann-Maree CatanzaritiJeffrey G Ellis
Aug 19, 2007·FEMS Microbiology Reviews·Anna M Łasica, Elzbieta K Jagusztyn-Krynicka
Dec 21, 2007·Biochimica Et Biophysica Acta·Christian Appenzeller-Herzog, Lars Ellgaard
Feb 10, 2009·Nature Reviews. Microbiology·Begoña HerasJennifer L Martin
Apr 4, 2009·PLoS Pathogens·Stephanie Abromaitis, Richard S Stephens
May 30, 2009·Antioxidants & Redox Signaling·Feras Hatahet, Lloyd W Ruddock
Feb 5, 2010·The Plant Journal : for Cell and Molecular Biology·Brendan S KelleyJocelyn K C Rose
Jul 1, 2004·Molecular Plant Pathology·Weixing ShanAdrienne R Hardham
Aug 13, 2011·Cellular & Molecular Biology Letters·Carlo TuranoSilvia Chichiarelli
Nov 30, 2011·TheScientificWorldJournal·Beatriz S StolfCelio X C Santos
Dec 19, 2012·Human Genomics·James J Galligan, Dennis R Petersen
Jul 8, 2014·Mycology·Yuling MengWeixing Shan
Mar 26, 2015·Plant Science : an International Journal of Experimental Plant Biology·Brad W PorterDavid A Christopher
Jul 1, 2003·The New Phytologist·Ralf T Voegele, Kurt Mendgen

❮ Previous
Next ❯

Citations

Jan 17, 2017·Fungal Genetics and Biology : FG & B·Mariana SchusterRegine Kahmann
May 13, 2020·The New Phytologist·Petra C BoevinkStephen C Whisson
May 19, 2017·Molecular Plant Pathology·Adrienne R Hardham, Leila M Blackman
Dec 20, 2020·International Journal of Molecular Sciences·Zhongling TianJingwu Zheng
Jul 27, 2021·Frontiers in Microbiology·Kévin GazengelStéphanie Daval

❮ Previous
Next ❯

Datasets Mentioned

BETA
CF891677

Methods Mentioned

BETA
protein folding
PCR

Software Mentioned

Clustal X

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.