The protein phosphatase-1 regulator NIPP1 is also a splicing factor involved in a late step of spliceosome assembly.

The Journal of Biological Chemistry
M Beullens, Mathieu Bollen

Abstract

NIPP1 is a ubiquitous regulator of protein phosphatase-1 (PP1) and is targeted to the splicing factor storage sites (speckles) in the nucleus by its forkhead-associated domain. We show here that NIPP1 is also a component of the spliceosomes in HeLa cell-splicing extracts and that the interaction with the spliceosomes requires a functional forkhead-associated domain. The in vitro splicing of beta-globin pre-mRNA was not affected by exogenous wild type NIPP1 but was blocked by mutants that lacked residues 225-329. The inhibition by these dominant negative mutants resulted from a block in a late phase of spliceosome assembly, i.e. at the transition between the B-complex and the C-complex. Site-directed mutagenesis furthermore showed that this spliceosomal function of NIPP1 was unrelated to its ability to bind PP1 or RNA. Our data suggest that NIPP1 can function independently as a splicing factor and a phosphatase regulator.

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Citations

Feb 7, 2008·Journal of Biomolecular NMR·Hiroyuki KumetaFuyuhiko Inagaki
Sep 2, 2004·Nature Reviews. Molecular Cell Biology·Chanseok Shin, James L Manley
Feb 24, 2007·Nature Reviews. Molecular Cell Biology·Greg B G MoorheadAnnegret Ulke-Lemée
Jun 2, 2009·Molecular & Cellular Proteomics : MCP·Michael KoflerChristian Freund
Jun 17, 2004·Molecular and Cellular Biology·Aleyde Van EyndeMathieu Bollen
Jun 8, 2012·PloS One·Stephen C ParnellJames P Calvet
Apr 25, 2015·FEBS Letters·Nele Van DesselMathieu Bollen
Nov 18, 2008·Journal of Molecular Biology·Simon TrowitzschMarkus C Wahl
Sep 7, 2007·Biochimica Et Biophysica Acta·Nivedita RoyMathieu Bollen
Mar 21, 2007·Trends in Plant Science·Ilona FarkasCsaba Koncz
Sep 26, 2013·Biochemical and Biophysical Research Communications·Malek KamounBrian E Wadzinski
Jan 16, 2004·Molecular & Cellular Proteomics : MCP·Hue T TranGreg B G Moorhead
Jun 15, 2018·Journal of Cell Science·Claudia WinklerMathieu Bollen
Jun 6, 2003·The Journal of Biological Chemistry·Tatyana AmmosovaSergei Nekhai
Jan 13, 2004·Physiological Reviews·Hugo Ceulemans, Mathieu Bollen
Sep 22, 2006·The Biochemical Journal·Paulina WakulaMathieu Bollen
Nov 21, 2007·The Journal of Biological Chemistry·Stefan Stamm
Jul 10, 2002·The Journal of Biological Chemistry·An BoudrezMathieu Bollen
Aug 21, 2003·Biochemical and Biophysical Research Communications·Deana LeonardRandy J Legerski

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