PMID: 11607454Feb 1, 1994Paper

The protein phosphatase inhibitor calyculin A mimics elicitor action in plant cells and induces rapid hyperphosphorylation of specific proteins as revealed by pulse labeling with [33P]phosphate

Proceedings of the National Academy of Sciences of the United States of America
G FelixT Boller

Abstract

Suspension-cultured tomato cells react to microbial signals, so-called elicitors, with rapid alkalinization of the growth medium and increased biosynthesis of the stress hormone ethylene. These responses to elicitors can be blocked by staurosporine and K-252a, two specific inhibitors of protein kinases. Here we show that calyculin A, a potent inhibitor of protein phosphatases, mimics the action of elicitors and, at nanomolar concentrations, induces medium alkalinization as well as a strong increase in the activity of 1-aminocyclopropane-1-carboxylate synthase, the key enzyme of ethylene biosynthesis. Both responses were strongly inhibited by K-252a, and calyculin A induced both responses more rapidly than did a fungal elicitor, xylanase. For example, the lag phase for medium alkalinization was only 0.2-0.4 min for calyculin A, compared with 2 min for xylanase. To study changes in the dynamics of protein phosphorylation, cells were labeled with 30-sec pulses of [33P]orthophosphate. Calyculin A strongly increased phosphorylation of several polypeptide bands within 40 sec of treatment. The same phosphorylated bands also appeared in response to xylanase, but only after a lag phase of 2-3 min. These results show that the protein pho...Continue Reading

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Citations

Mar 6, 1999·Current Opinion in Plant Biology·D Scheel
Nov 11, 1998·Comptes rendus de l'Académie des sciences. Série III, Sciences de la vie·D PontierD Roby
Apr 17, 1999·The Plant Journal : for Cell and Molecular Biology·A TakahashiK Shimamoto
Aug 6, 2000·The Plant Journal : for Cell and Molecular Biology·S ZhangD F Klessig
Jul 29, 2010·Proceedings of the National Academy of Sciences of the United States of America·Freddy BoutrotJohn P Rathjen
May 9, 1995·Proceedings of the National Academy of Sciences of the United States of America·S Chandra, P S Low
May 9, 1995·Proceedings of the National Academy of Sciences of the United States of America·H Sano, Y Ohashi
May 9, 1995·Proceedings of the National Academy of Sciences of the United States of America·P ReymondE E Farmer
Jun 1, 1996·Annual Review of Plant Physiology and Plant Molecular Biology·Robert D. Smith, John C. Walker
Jun 1, 1997·Annual Review of Plant Physiology and Plant Molecular Biology·Chris Lamb, Richard A. Dixon
Jun 24, 1998·Proceedings of the National Academy of Sciences of the United States of America·S Zhang, D F Klessig
Aug 2, 2000·Proceedings of the National Academy of Sciences of the United States of America·J StratmannC A Ryan
Mar 13, 2012·Annual Review of Plant Biology·Benjamin Schwessinger, Pamela C Ronald
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Jan 26, 2008·The Journal of Biological Chemistry·Yoko OgasawaraKazuyuki Kuchitsu
Jul 21, 2020·Current Opinion in Biotechnology·Dongqi WangApril Z Gu

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