PMID: 7544867Apr 12, 1995Paper

The proteoglycan perlecan is expressed in the erythroleukemia cell line K562 and is upregulated by sodium butyrate and phorbol ester

Molecular and Cellular Biochemistry
S GrässelR V Iozzo

Abstract

Perlecan is a modular heparan sulfate proteoglycan that harbors five domains with homology to the low density lipoprotein receptor, epidermal growth factor, laminin and neural cell adhesion molecule. Using a monoclonal antibody directed against the laminin-like domain of perlecan, we have recently shown that perlecan is widely expressed in all lymphoreticular systems. To investigate further this observation we have studied the expression of perlecan in two human leukemic cell lines. Using reverse transcriptase-PCR, ribonuclease protection assay, and metabolic labeling we detected significant perlecan expression in the multipotential cell line K562, originally derived from a patient with chronic myelogenous leukemia. In contrast, the promyelocytic cell line HL-60 expressed perlecan at barely detectable levels. These results were intriguing because the K562 cells do not assemble or produce a classical basement membrane. Following induction with either sodium butyrate or the phorbol diester 12-0-tetradecanoylphorbol-13-acetate (TPA), K562 and HL-60 differentiate into early progenitor cells with erythroid or megakaryocytic properties, respectively. Following treatment of K562 and HL-60 cells with either of these agents, perlecan ex...Continue Reading

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Citations

Feb 14, 1998·Journal of Cellular Physiology·M S FillaA C Rapraeger
Oct 6, 1998·Annual Review of Biochemistry·R V Iozzo
Mar 8, 2013·PloS One·Shun-lung FangMargaret Dah-Tsyr Chang
Jul 17, 2015·Connective Tissue Research·Stephen DouglassRenato V Iozzo
Jun 7, 2005·British Journal of Pharmacology·Simon W JonesMark A Lindsay
Jul 17, 2008·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Kathryn D RodgersOlena Jacenko
Mar 21, 1998·The Journal of Biological Chemistry·B Sharma, R V Iozzo
Oct 23, 1997·Developmental Dynamics : an Official Publication of the American Association of Anatomists·M HandlerR V Iozzo
Dec 26, 2006·The Journal of Biological Chemistry·Karen CuellarMyron E Hinsdale
Aug 15, 1998·Biochemical and Biophysical Research Communications·J A Rivero, S E Adunyah
Dec 16, 2020·Blood Reviews·Ishnoor SidhuAnilkumar Gopalakrishnapillai

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