Abstract
Perlecan is a modular heparan sulfate proteoglycan that harbors five domains with homology to the low density lipoprotein receptor, epidermal growth factor, laminin and neural cell adhesion molecule. Using a monoclonal antibody directed against the laminin-like domain of perlecan, we have recently shown that perlecan is widely expressed in all lymphoreticular systems. To investigate further this observation we have studied the expression of perlecan in two human leukemic cell lines. Using reverse transcriptase-PCR, ribonuclease protection assay, and metabolic labeling we detected significant perlecan expression in the multipotential cell line K562, originally derived from a patient with chronic myelogenous leukemia. In contrast, the promyelocytic cell line HL-60 expressed perlecan at barely detectable levels. These results were intriguing because the K562 cells do not assemble or produce a classical basement membrane. Following induction with either sodium butyrate or the phorbol diester 12-0-tetradecanoylphorbol-13-acetate (TPA), K562 and HL-60 differentiate into early progenitor cells with erythroid or megakaryocytic properties, respectively. Following treatment of K562 and HL-60 cells with either of these agents, perlecan ex...Continue Reading
References
Jul 12, 1979·Nature·T R RutherfordD J Weatherall
Feb 1, 1979·International Journal of Cancer. Journal International Du Cancer·L C AnderssonC G Gahmberg
Mar 22, 1979·Nature·L C AnderssonC G Gahmberg
Jun 14, 1979·Nature·M JokinenL C Andersson
Feb 1, 1992·Matrix : Collagen and Related Research·K G DanielsonR V Iozzo
Dec 1, 1987·The Biochemical Journal·G LorkowskiA Hasilik
Nov 15, 1986·The Biochemical Journal·D J ThorntonJ K Sheehan
Aug 1, 1984·The Journal of Cell Biology·R V Iozzo
Apr 1, 1981·Proceedings of the Society for Experimental Biology and Medicine·B B LozzioA S Feliu
Feb 1, 1994·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·A D MurdochR V Iozzo
Apr 1, 1994·Matrix Biology : Journal of the International Society for Matrix Biology·R V Iozzo
Sep 15, 1994·The Biochemical Journal·R V IozzoA D Murdoch
Nov 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·I R CohenR V Iozzo
Aug 1, 1993·Genes & Development·T M RogalskiD G Moerman
Citations
Aug 26, 1998·Journal of Cellular Biochemistry·M A PorcionattoH B Nader
Feb 14, 1998·Journal of Cellular Physiology·M S FillaA C Rapraeger
Sep 18, 2001·Biomedical Chromatography : BMC·E MakatsoriT Tsegenidis
Oct 6, 1998·Annual Review of Biochemistry·R V Iozzo
Mar 8, 2013·PloS One·Shun-lung FangMargaret Dah-Tsyr Chang
Jul 17, 2015·Connective Tissue Research·Stephen DouglassRenato V Iozzo
Jun 7, 2005·British Journal of Pharmacology·Simon W JonesMark A Lindsay
Jul 17, 2008·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Kathryn D RodgersOlena Jacenko
Mar 21, 1998·The Journal of Biological Chemistry·B Sharma, R V Iozzo
Oct 23, 1997·Developmental Dynamics : an Official Publication of the American Association of Anatomists·M HandlerR V Iozzo
Apr 19, 2006·Proteomics·Meiping WuYingnian Yu
Apr 16, 2008·Tsitologiia·N A FilatovaV A Ivanov
Oct 23, 1997·Glia·J D MillerA D Snow
Sep 12, 2001·The Journal of Biological Chemistry·Z ZhangG David
Feb 21, 1997·The Journal of Biological Chemistry·R V IozzoA Mauviel
Dec 26, 2006·The Journal of Biological Chemistry·Karen CuellarMyron E Hinsdale
Dec 25, 2012·Molecular Biology Reports·Weimin WangBang Liu
Jul 2, 2020·Cell Death & Disease·Xiaojia ZhouLi Wang
Aug 15, 1998·Biochemical and Biophysical Research Communications·J A Rivero, S E Adunyah
Nov 20, 1998·Life Sciences·P R Pouillart
Dec 16, 2020·Blood Reviews·Ishnoor SidhuAnilkumar Gopalakrishnapillai