The proteolipid of the A(1)A(0) ATP synthase from Methanococcus jannaschii has six predicted transmembrane helices but only two proton-translocating carboxyl groups.

The Journal of Biological Chemistry
C RuppertV Müller

Abstract

The proteolipid, a hydrophobic ATPase subunit essential for ion translocation, was purified from membranes of Methanococcus jannaschii by chloroform/methanol extraction and gel chromatography and was studied using molecular and biochemical techniques. Its apparent molecular mass as determined in SDS-polyacrylamide gel electrophoresis varied considerably with the conditions applied. The N-terminal sequence analysis made it possible to define the open reading frame and revealed that the gene is a triplication of the gene present in bacteria. In some of the proteolipids, the N-terminal methionine is excised. Consequently, two forms with molecular masses of 21,316 and 21,183 Da were determined by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The molecular and biochemical data gave clear evidence that the mature proteolipid from M. jannaschii is a triplication of the 8-kDa proteolipid present in bacterial F(1)F(0) ATPases and most archaeal A(1)A(0) ATPases. Moreover, the triplicated form lacks a proton-translocating carboxyl group in the first of three pairs of transmembrane helices. This finding puts in question the current view of the evolution of H(+) ATPases and has important mechanistic consequen...Continue Reading

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Citations

May 13, 2006·Journal of Bioenergetics and Biomembranes·V MüllerMichael Fritz
Nov 7, 2003·Applied and Environmental Microbiology·Volker Müller
Apr 29, 2006·Journal of Molecular Microbiology and Biotechnology·V MüllerGerhard Grüber
Apr 26, 2001·Proceedings of the National Academy of Sciences of the United States of America·W JiangR H Fillingame
Oct 15, 2013·FEMS Microbiology Reviews·Florian Mayer, Volker Müller
Jun 30, 2006·Biochimica Et Biophysica Acta·Kim Lewalter, V Müller
Apr 12, 2001·FEBS Letters·I Arechaga, P C Jones
May 28, 2003·Extremophiles : Life Under Extreme Conditions·Astrid LinglV Müller
Sep 5, 2001·Nature Reviews. Molecular Cell Biology·M YoshidaT Hisabori
Jan 19, 2021·Biochimica Et Biophysica Acta. Bioenergetics·Lars WestphalVolker Müller
Mar 12, 2021·Biochemistry. Biokhimii︠a︡·V M ZubarevaB A Feniouk

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