The proteolipid protein promoter drives expression outside of the oligodendrocyte lineage during embryonic and early postnatal development.

PloS One
John-Paul MichalskiRashmi Kothary

Abstract

The proteolipid protein (Plp) gene promoter is responsible for driving expression of one of the major components of myelin--PLP and its splice variant DM-20. Both products are classically thought to express predominantly in oligodendrocytes. However, accumulating evidence suggests Plp expression is more widespread than previously thought. In an attempt to create a mouse model for inducing oligodendrocyte-specific gene deletions, we have generated transgenic mice expressing a Cre recombinase cDNA under control of the mouse Plp promoter. We demonstrate Plp promoter driven Cre expression is restricted predominantly to mature oligodendrocytes of the central nervous system (CNS) at postnatal day 28. However, crosses into the Rosa26(LacZ) and mT/mG reporter mouse lines reveal robust and widespread Cre activity in neuronal tissues at E15.5 and E10.5 that is not strictly oligodendrocyte lineage specific. By P28, all CNS tissues examined displayed high levels of reporter gene expression well outside of defined white matter zones. Importantly, our study reinforces the emerging idea that Plp promoter activity is not restricted to the myelinating cell lineage, but rather, has widespread activity both during embryonic and early postnatal de...Continue Reading

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Citations

Sep 29, 2011·Genesis : the Journal of Genetics and Development·Naoko InamuraKazuhiro Ikenaka
Dec 14, 2011·Glia·Frank W Pfrieger, Michal Slezak
Jul 3, 2013·Pigment Cell & Melanoma Research·Catherine D Van Raamsdonk, Mugdha Deo
Jun 25, 2015·Frontiers in Neuroanatomy·Badrah Alghamdi, Robert Fern
May 5, 2016·Cellular and Molecular Life Sciences : CMLS·Hande OzgenNicoletta Kahya
Aug 11, 2019·Journal of Neuropathology and Experimental Neurology·Raymond A SobelJulian R Hinojoza
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May 1, 2020·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Shujing GuoAijun Wang
Nov 16, 2019·Cells·Sarah KuhnYvonne Dombrowski
Sep 27, 2020·The Journal of Biological Chemistry·Michael SyMichael Demetriou
Jan 18, 2021·The Journal of Biological Chemistry·Michael SyMichael Demetriou
Jul 3, 2021·Cells·Ivó H HernándezMaría José Pérez-Álvarez
Jul 25, 2021·International Journal of Molecular Sciences·Federica CherchiElisabetta Coppi

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Methods Mentioned

BETA
genetic modifications
biopsies
transgenic
PCR
fluorescence microscopy

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