PMID: 9525644Apr 3, 1998Paper

The proteolytic cleavage of human immunodeficiency virus type 1 Nef does not correlate with its ability to stimulate virion infectivity

Journal of Virology
Y L ChenD Camaur

Abstract

The Nef protein of human immunodeficiency virus type 1 (HIV-1) promotes virion infectivity through mechanisms that are yet ill defined. Some Nef is incorporated into particles, where it is cleaved by the viral protease between amino acids 57 and 58. The functional significance of this event, which liberates the C-terminal core domain of the protein from its membrane-associated N terminus, is unknown. To address this question, we examined the modalities of Nef virion association and processing. We found that although significant levels of Nef were detected in HIV-1 virions partly in a cleaved form, cell-specific variations existed in the efficiency of Nef proteolytic processing. The virion association of Nef was strongly enhanced by myristoylation but did not require other HIV-1-specific proteins, since Nef was efficiently incorporated into and cleaved inside murine leukemia virus particles. Substituting alanine for tryptophan57 decreased the efficiency of Nef processing, while mutating leucine58 had little effect. In contrast, replacing both of these residues simultaneously almost completely prevented this process. However, when the resulting mutants were compared with a wild-type control in viral infectivity assays, no correla...Continue Reading

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Citations

Sep 13, 2003·Journal of Virology·Minoru TobiumeChristopher Aiken
Aug 22, 2008·Journal of Virology·Massimo PizzatoHeinrich G Göttlinger
Jun 7, 2002·Journal of Virology·Tatyana DorfmanHeinrich G Göttlinger
Jul 13, 2000·Protein Science : a Publication of the Protein Society·S AroldC Dumas
Jun 7, 2014·Frontiers in Microbiology·Stéphane Basmaciogullari, Massimo Pizzato
Jul 12, 2016·Viruses·Marcela Sabino CunhaLuciana Jesus da Costa
Jul 1, 1999·Reviews in Medical Virology·V Piguet, D Trono
Jul 1, 2016·Microbiology and Molecular Biology Reviews : MMBR·Guangdi Li, Erik De Clercq
Nov 7, 2008·Journal of Virology·Nadine LaguetteStéphane Basmaciogullari
May 26, 2004·AIDS Research and Human Retroviruses·Angela CiuffiPascal R A Meylan
Sep 11, 1999·Journal of Virology·A KotovC Aiken
Nov 1, 2017·Scientific Reports·R Charlotte EcclestonNeil Dalchau
Apr 1, 2021·The Biochemical Journal·Abdullah AldehaimanStefan T Arold
Aug 13, 2005·Molecular Therapy : the Journal of the American Society of Gene Therapy·Silvia PerettiMaurizio Federico
May 11, 2006·Virology·Oliver T FacklerHans-Georg Kräusslich
Jul 3, 2010·Molecular Aspects of Medicine·Nadine LaguetteStéphane Basmaciogullari

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