PMID: 9431985Feb 7, 1998Paper

The proteolytic cleavage of protein kinase C isotypes, which generates kinase and regulatory fragments, correlates with Fas-mediated and 12-O-tetradecanoyl-phorbol-13-acetate-induced apoptosis

European Journal of Biochemistry
K MizunoS Ohno

Abstract

Protein kinase C (PKC) has been implicated in signaling induced by diverse sets of stimuli regulating growth, differentiation, and apoptosis. The present study focused on the fate of PKC isotype proteins during Fas-mediated apoptosis of human leukemic cell lines. Among the PKC isotypes expressed in different cell types, such as Jurkat, HPB-ALL, U937, and HL60, all the nPKC isotypes including nPKCdelta, nPKC epsilon, and nPKCtheta, but not cPKC alpha and betaII and aPKCzeta (n, c, and a represent novel, conventional and atypical, respectively), showed limited proteolytic cleavage during Fas-mediated apoptosis. The limited proteolysis of nPKC isotypes means the disappearance of the intact protein band concomitant with the appearance of two fragments, most likely containing the kinase and regulatory domains, in contrast to the so-called down-regulation known for both cPKC and nPKC isotypes following exposure to stimuli such as 12-O-tetradecanoyl-phorbol 13-acetate (TPA). The time course of Fas-mediated apoptosis in Jurkat cells parallels that of the activation of a 32-kDa cysteine protease (CPP32)-like protease and also closely parallels the proteolytic cleavage of nPKC isotypes. A peptide inhibitor of the CPP32-like protease, Ac-...Continue Reading

References

Mar 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·E Huberman, M F Callaham
Nov 1, 1992·Cellular Signalling·J J SandoC M Grisham
Dec 18, 1991·European Journal of Biochemistry·K MizunoK Suzuki
May 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·S PontremoliB L Horecker
Mar 24, 1995·The Journal of Biological Chemistry·S J MartinD R Green
Sep 25, 1995·Biochemical and Biophysical Research Communications·W ZhuC Y Young
Apr 1, 1995·Molecular Biology of the Cell·G J Den HaeseK L Gould
Nov 1, 1994·European Journal of Pediatrics·J RajantieM Lanning
Oct 1, 1994·European Journal of Biochemistry·G BaierA Altman
Feb 1, 1994·Trends in Biochemical Sciences·L V Dekker, P J Parker
May 1, 1994·Journal of Cellular Physiology·M K SquìerJ J Cohen
Jun 14, 1996·Cell·A Fraser, G Evan
Oct 18, 1996·Cell·E S AlnemriJ Yuan
May 16, 1997·International Journal of Cancer. Journal International Du Cancer·Y MasudaK Nakaya
Nov 1, 1996·Trends in Cell Biology·M Whyte

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Citations

Apr 22, 2005·Apoptosis : an International Journal on Programmed Cell Death·W LeeI Mook-Jung
May 16, 2006·Apoptosis : an International Journal on Programmed Cell Death·C LautretteM O Jauberteau
Jan 27, 2007·Digestive Diseases and Sciences·Maria J RedlakThomas A Miller
Oct 4, 2006·Nephrology·Jun Li, Glenda Gobe
Oct 17, 2008·Physiological Reviews·Susan F Steinberg
Jan 26, 2013·The Journal of Clinical Investigation·Motofumi KumazoeHirofumi Tachibana
Nov 22, 2001·Biochemical and Biophysical Research Communications·M FukunagaU Kikkawa
Oct 30, 2013·Journal of Cancer Research and Therapeutics·Chenfang FanJack Jia
Sep 18, 2001·Biological & Pharmaceutical Bulletin·Y SakuraiH Kizaki
Dec 7, 2000·Cancer Investigation·L Cartee, G L Kucera
Aug 12, 2004·The Journal of Investigative Dermatology·Leonid A SitailoMitchell F Denning
Feb 3, 2004·Journal of Cellular and Molecular Medicine·Alakananda Basu
Mar 4, 2008·The European Journal of Neuroscience·Katharine HanrottSusan Wonnacott
Jan 15, 2005·Biochemical and Biophysical Research Communications·Goro YokoyamaKazuo Shirouzu
Mar 21, 2003·Biochemical and Biophysical Research Communications·Miyoko MatsumuraMotoi Ohba
Mar 31, 2000·Experimental Cell Research·T G CrossJ M Lord
Apr 2, 2015·Biochimica Et Biophysica Acta·Roberta GonnellaMara Cirone
Mar 5, 2013·Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology·Hsueh-Chun WangHung-Che Chiang
Nov 30, 2004·Cellular Signalling·Ajit Sodhi, Gautam Sethi
Aug 12, 2006·The Journal of Biological Chemistry·Leonid A SitailoMitchell F Denning
Oct 10, 2002·Toxicologic Pathology·Mireia Gómez-Angelats, John A Cidlowski
Nov 25, 2003·Oncogene·Rosario RizzutoTullio Pozzan
Apr 24, 2004·The Journal of Biological Chemistry·Robert V StahelinWonhwa Cho
Mar 26, 2003·Cell Death and Differentiation·U FischerK Schulze-Osthoff
Aug 24, 2004·The Journal of Biological Chemistry·Tracie A DeVriesMary E Reyland

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