The proteome of Salmonella Typhimurium grown under in vivo-mimicking conditions

Proteomics
Kathleen A J SonckSigrid C J De Keersmaecker

Abstract

To successfully infect a host, it is a prerequisite for enteric pathogens such as Salmonella enterica serovar Typhimurium to adapt to their environment, in casu the gastrointestinal tract. The adoption of an appropriate lifestyle is triggered by environmental signals such as the low oxygen availability and high osmolarity prevalent in the gut. In order to gain more insight in the changes that are induced when S. Typhimurium is adapting to these particular conditions, we used 2-D DIGE technology to investigate the combined effect of low oxygen tension and high osmolarity on the proteome of S. Typhimurium SL1344 compared to standard laboratory conditions. As a validation of the 2-D DIGE technique, preferential protein labeling by the Cy-dyes was assessed and proved to be negligible. The differentially expressed proteins identified reflect very well the applied culture conditions. Furthermore, reported transcriptional changes and observed changes at the translational level show overlap. Among the metabolic processes that are upregulated under in vivo-mimicking conditions are anaerobic fumarate respiration and the utilization of 1,2-propanediol. We also provide evidence that S. Typhimurium expresses an arginine deiminase pathway fo...Continue Reading

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Citations

Nov 3, 2010·Applied and Environmental Microbiology·Shabarinath Srikumar, Thilo M Fuchs
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