PMID: 9183018May 1, 1997Paper

The prothoracicotropic hormone bombyxin has specific receptors on insect ovarian cells

European Journal of Biochemistry
G FullbrightE E Büllesbach

Abstract

Bombyxin II, a product of the brain of the adult silkmoth, Bombyx mori, binds to ovarian cells of three different species of lepidoptera, i.e. B. mori (silkmoth), Samia cynthia ricini (ailanthus moth), and an ovarian cell line of Spodoptera frugiperda (Sf9) (fall armyworm). Crude Sf9 cell membrane preparations were used to show that the purported bombyxin receptor binds its ligand in a specific, saturable, and reversible manner. The dissociation constant of the bombyxin-receptor complex is 260+/-90 pM. Quantitative binding studies and Scatchard analysis suggest that every Sf9 cell displays 20000 receptors on the surface. The cross-linked bombyxin-receptor ligand complex has an apparent molecular mass of about 300 kDa as determined by SDS/PAGE. Reduction causes the bombyxin receptor to dissociate into two subunits with molecular masses of 90 kDa and 116 kDa. The size and subunit structure of the putative bombyxin receptor on Sf9 cells show some similarities to the mammalian insulin receptor.

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Citations

Nov 6, 2003·Insect Biochemistry and Molecular Biology·Luc Swevers, Kostas Iatrou
Mar 19, 2002·Peptides·Ilse ClaeysJozef Vanden Broeck
Oct 21, 1999·Insect Biochemistry and Molecular Biology·M A Riehle, M R Brown
Aug 17, 1999·The Journal of Peptide Research : Official Journal of the American Peptide Society·E E Büllesbach
Apr 9, 2008·Proceedings of the National Academy of Sciences of the United States of America·Mark R BrownMichael R Strand
Dec 8, 2005·Annual Review of Entomology·Qi Wu, Mark R Brown
Jun 2, 2005·In Vitro Cellular & Developmental Biology. Animal·Shintaro GotoMakio Takeda
Jan 7, 2010·Journal of Insect Science·William H Telfer
Aug 6, 2008·Annual Review of Entomology·Mark R BrownHuw H Rees
Nov 15, 2011·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Xanthe VafopoulouColin G H Steel
Dec 17, 2008·General and Comparative Endocrinology·Heleen VerlindenJozef Vanden Broeck
Dec 4, 2008·Insect Biochemistry and Molecular Biology·Shi-Hong GuChien-Hung Chen
Sep 2, 2008·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Shinji NagataHiromichi Nagasawa
Jun 15, 2007·General and Comparative Endocrinology·Sandrien Van de VeldeGuy Smagghe
Nov 8, 2005·Journal of Insect Physiology·Yuichi NakaharaManabu Kamimura
Feb 24, 2017·International Journal of Molecular Sciences·Bi-Yue DingJin-Jun Wang
May 2, 2017·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Kazuhisa Sekimizu
Aug 10, 2000·Biochemistry·G Fullbright, E E Büllesbach

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