The proto-oncogene c-fos mediates apoptosis in murine T-lymphocytes induced by ionizing radiation and dexamethasone

Biochemical and Biophysical Research Communications
M PruschyS Bodis

Abstract

Expression of the immediate early response gene c-fos is induced by several cellular and extracellular stress factors including ionizing radiation. We examined the role of c-fos in mediating stress-induced apoptosis of isogenic CD4+ and CD8+ mouse T-lymphocytes differing only in their c-fos status after treatment with ionizing radiation and the synthetic glucocorticoid dexamethasone. The amount of radiation-induced apoptosis was decreased (up to 37%) in the T-lymphocyte population derived from the knockout mice lacking endogenous c-fos compared to the wildtype T-lymphocyte population. The difference in apoptosis induction in T-lymphocytes from wildtype and c-fos knockout mice was even more prominent (up to 55%) after dexamethasone treatment. Comparative experiments were performed with T-lymphocytes from isogenic mouse littermates differing only in the status of the tumor-suppressor gene p53. Whereas p53 plays a primary role in radiation-induced apoptosis, our results suggest that c-fos enhances both p53-dependent radiation- and p53-independent steroid-induced apoptosis in T-lymphocytes.

Citations

Feb 18, 2011·Leukemia & Lymphoma·Elena KanDaniel Benharroch
Jul 27, 2011·Environmental Toxicology and Pharmacology·Tanvisith CharoenyingJutamaad Satayavivad
Jan 22, 2005·Annals of the New York Academy of Sciences·Jeung Hee AnJinsil Seong
Sep 19, 2009·Virchows Archiv : an International Journal of Pathology·Elena KanDaniel Benharroch
May 30, 2003·Journal of Leukocyte Biology·Seetha M Lakshmi Tamma, Richard F Coico
Mar 19, 1999·Molecular and Cellular Biology·A ElkelesM Oren
Jun 1, 2002·Molecular and Cellular Endocrinology·Abraham Amsterdam, Ravid Sasson

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis