The prototype foamy virus protease is active independently of the integrase domain.

Retrovirology
Ralf SpannausJochen Bodem

Abstract

Recently, contradictory results on foamy virus protease activity were published. While our own results indicated that protease activity is regulated by the viral RNA, others suggested that the integrase is involved in the regulation of the protease. To solve this discrepancy we performed additional experiments showing that the protease-reverse transcriptase (PR-RT) exhibits protease activity in vitro and in vivo, which is independent of the integrase domain. In contrast, Pol incorporation, and therefore PR activity in the viral context, is dependent on the integrase domain. To further analyse the regulation of the protease, we incorporated Pol in viruses by expressing a GagPol fusion protein, which supported near wild-type like infectivity. A GagPR-RT fusion, lacking the integrase domain, also resulted in wild-type like Gag processing, indicating that the integrase is dispensable for viral Gag maturation. Furthermore, we demonstrate with a trans-complementation assays that the PR in the context of the PR-RT protein supports in trans both, viral maturation and infectivity. We provide evidence that the FV integrase is required for Pol encapsidation and that the FV PR activity is integrase independent. We show that an active PR ca...Continue Reading

References

Apr 30, 1996·Proceedings of the National Academy of Sciences of the United States of America·J EnssleA Rethwilm
Nov 22, 2000·The Journal of General Virology·H ImrichA Rethwilm
Mar 22, 2002·Journal of Virology·Martin HeinkeleinAxel Rethwilm
Aug 12, 2003·Current Topics in Microbiology and Immunology·M Löchelt
Jul 4, 2008·Journal of Molecular Biology·Maximilian J HartlKristian Schweimer
Feb 9, 2010·The Biochemical Journal·Maximilian J HartlBirgitta M Wöhrl
Dec 17, 2010·Journal of Virology·Jochen BodemAxel Rethwilm
Feb 18, 2011·Journal of Virology·Maximilian J HartlBirgitta M Wöhrl
Oct 14, 2011·Viruses·Dirk Lindemann, Axel Rethwilm

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Citations

Mar 23, 2013·Viruses·Eun-Gyung LeeMaxine L Linial
Sep 28, 2013·Viruses·Axel Rethwilm, Jochen Bodem
Oct 15, 2013·Current Opinion in Virology·Axel Rethwilm
Jan 17, 2021·Viruses·Dirk LindemannMartin Löchelt

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Methods Mentioned

BETA
electrophoresis
transfection
transfections
PCR

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