PMID: 15220199Jun 29, 2004Paper

The proximal islet-specific glucose-6-phosphatase catalytic subunit-related protein autoantigen promoter is sufficient to initiate but not maintain transgene expression in mouse islets in vivo

Diabetes
C FrigeriRichard M O'Brien

Abstract

We have previously reported the discovery of an islet-specific glucose-6-phosphatase catalytic subunit-related protein (IGRP) that is predominantly expressed in islet beta-cells. IGRP has recently been identified as a major autoantigen in a mouse model of type 1 diabetes. The analysis of IGRP-chloramphenicol acetyltransferase (CAT) fusion gene expression in transiently transfected islet-derived hamster insulinoma tumor and betaTC-3 cells revealed that the promoter region located between -306 and +3 confers high-level reporter gene expression. To determine whether this same promoter region is sufficient to confer islet beta-cell-specific gene expression in vivo, it was ligated to a beta-galactosidase reporter gene, and transgenic mice expressing the resulting fusion gene were generated. In two independent founder lines, this -306 to +3 promoter region was sufficient to drive beta-galactosidase expression in newborn mouse islets, predominantly in beta-cells, which was initiated during the expected time in development, around embryonic day 12.5. However, unlike the endogenous IGRP gene, beta-galactosidase expression was also detected in the cerebellum. Moreover, beta-galactosidase expression was almost completely absent in adult m...Continue Reading

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Citations

Aug 25, 2009·The Journal of Biological Chemistry·John C Hutton, Richard M O'Brien
Nov 19, 2010·Medicine and Science in Sports and Exercise·Sheri R ColbergUNKNOWN American Diabetes Association
Aug 30, 2008·Journal of Molecular Endocrinology·Cyrus C MartinRichard M O'Brien
Apr 29, 2005·Diabetes/metabolism Research and Reviews
Jan 27, 2017·Journal of Molecular Endocrinology·Kayla A BoortzRichard M O'Brien

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