PMID: 9421524Feb 28, 1998Paper

The proximal sequence element (PSE) plays a major role in establishing the RNA polymerase specificity of Drosophila U-snRNA genes

Nucleic Acids Research
R C JensenWilliam E Stumph

Abstract

Most small nuclear RNA (snRNA) genes are transcribed by RNA polymerase II, but some (e.g., U6) are transcribed by RNA polymerase III. In vertebrates a TATA box at a fixed distance downstream of the proximal sequence element (PSE) acts as a dominant determinant for recruiting RNA polymerase III to U6 gene promoters. In contrast, vertebrate snRNA genes that contain a PSE but lack a TATA box are transcribed by RNA polymerase II. In plants, transcription of both classes of snRNA genes requires a TATA box in addition to an upstream sequence element (USE), and polymerase specificity is determined by the spacing between these two core promoter elements. In these examples, the PSE (or USE) is interchangeable between the two classes of snRNA genes. Here we report the surprising finding that the Drosophila U1 and U6 PSEs cannot functionally substitute for each other; rather, determination of RNA polymerase specificity is an intrinsic property of the PSE sequence itself. The alteration of two or three base pairs near the 3'-end of the U1 and U6 PSEs was sufficient to switch the RNA polymerase specificity of Drosophila snRNA promoters in vitro. These findings reveal a novel mechanism for achieving RNA polymerase specificity at insect snRNA...Continue Reading

References

Jul 25, 1992·Nucleic Acids Research·B J Wendelburg, W F Marzluff
Dec 6, 1991·Science·J E Dahlberg, E Lund
Sep 12, 1995·Proceedings of the National Academy of Sciences of the United States of America·Y Wang, W E Stumph

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Citations

Mar 29, 2007·International Journal of Biological Sciences·Hongxia ZhouXu Gang Xia
Dec 18, 2002·Proceedings of the National Academy of Sciences of the United States of America·Anish Das, Vivian Bellofatto
Jun 24, 2004·Proceedings of the National Academy of Sciences of the United States of America·Claudia SchneiderReinhard Lührmann
Jul 23, 2003·Proceedings of the National Academy of Sciences of the United States of America·Zbigniew DominskiWilliam F Marzluff
Jul 26, 2000·The Journal of Cell Biology·S M Mount, H K Salz
Oct 12, 2010·Critical Reviews in Biochemistry and Molecular Biology·Ko-Hsuan Hung, William E Stumph
Aug 16, 2005·The Plant Journal : for Cell and Molecular Biology·Misato Ohtani, Munetaka Sugiyama
Jun 23, 2001·Journal of Molecular Biology·E P Geiduschek, G A Kassavetis
Mar 27, 2018·Plant Biotechnology Journal·Junhui ZhouZhongchi Liu
Oct 29, 2005·Human Molecular Genetics·Pia HermannsBrendan Lee
Jun 8, 2001·The Journal of Biological Chemistry·N Hernandez
Apr 8, 2020·Molecular and Cellular Biology·Mun Kyoung KimWilliam E Stumph
Dec 3, 2016·Journal of Plant Research·Misato Ohtani
Jan 9, 2021·RNA Biology·Yi RanChengguo Yao
Sep 1, 2021·BMC Molecular and Cell Biology·Duy NguyenPatricia Uguen

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