PMID: 7539498Feb 1, 1995Paper

The proximal straight tubule (PST) basolateral cell membrane water channel: selectivity characteristics

The Journal of Membrane Biology
A M GutiérrezG Whittembury

Abstract

Proximal straight tubules (PST) were dissected from rabbit kidneys, held by crimping pipettes in a chamber and bathed in a buffered isosmotic (295 mOsm/kg) solution containing 200 mM mannitol (MBS). Changes in tubule diameter were monitored on line with an inverted microscope, TV camera and image processor. The PST were then challenged for 20 sec with MBS made 35 mOsm/kg hyperosmotic by addition of either NaCl, KCl, mannitol (M), glycerol (G), ethylene glycol (E), glycine (g), urea (U), acetamide (A) or formamide (F). With NaCl, KCl, M, G, E, g, U, and A, tubules shrunk osmometrically within 0.5 sec and remained shrunk for as long as 20 sec without recovering their original volume (sometimes A showed some recovery). PST barely shrunk with F and quickly recovered their original volume. The permeability coefficients were 0 microns/sec (NaCl, M, g, E and U), 1 micron/sec (A), 84 microns/sec (F) and 0.02 micron/sec (G). The reflection coefficients sigma = 1.0 (NaCl, KCl, M, G, E, g and U), 0.95 (A) and 0.62 (F). Similar sigma values were obtained by substituting 200 mOsm/kg M in MBS by either NaCl, KCl, G, E, g, U, a or F. The olive oil/water partition coefficients are 5 (M), 15 (U), 85 (A) and 75 (F) (all x 10(-5)). Thus, part of ...Continue Reading

Citations

Jun 6, 1998·The Journal of Physiology·A MeinildT Zeuthen
May 4, 2004·Surgical Neurology·Donald E G Griesdale, Christopher R Honey
Jun 6, 1998·Journal of Structural Biology·J B HeymannA Engel
Mar 29, 2002·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Jérôme BadautLuca Regli
Nov 26, 1998·The Journal of Biological Chemistry·A K MeinildT Zeuthen
Feb 13, 2018·Acta Neuropathologica·N Joan AbbottRobert G Thorne
Feb 23, 2021·Reviews in the Neurosciences·Galina YankovaAndrey Tulupov
Jul 16, 2020·Journal of the American Chemical Society·Bo SunHua Chun Zeng

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