PMID: 8604353Mar 15, 1996Paper

The PRP31 gene encodes a novel protein required for pre-mRNA splicing in Saccharomyces cerevisiae

Nucleic Acids Research
E M WeidenhammerJ L Woolford

Abstract

The pre-mRNA splicing factor Prp31p was identified in a screen of temperature-sensitive yeast strains for those exhibiting a splicing defect upon shift to the non- permissive temperature. The wild-type PRP31 gene was cloned and shown to be essential for cell viability. The PRP31 gene is predicted to encode a 60 kDa polypeptide. No similarities with other known splicing factors or motifs indicative of protein-protein or RNA-protein interaction domains are discernible in the predicted amino acid sequence. A PRP31 allele bearing a triple repeat of the hemagglutinin epitope has been generated. The tagged protein is functional in vivo and a single polypeptide species of the predicted size was detected by Western analysis with proteins from yeast cell extracts. Functional Prp31p is required for the processing of pre-mRNA species both in vivo and in vitro, indicating that the protein is directly involved in the splicing pathway.

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Citations

Jul 22, 2008·Human Molecular Genetics·Thomas Rio FrioCarlo Rivolta
Mar 15, 1996·Nucleic Acids Research·J R MaddockJ L Woolford
Sep 2, 1998·The Plant Cell·R W Sablowski, E M Meyerowitz
Oct 1, 2003·Molecular and Cellular Biology·Qiang Wang, Brian C Rymond
Oct 7, 2003·Annual Review of Neuroscience·Laura R PacioneRoderick R McInnes
Jan 22, 2005·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Liya YuanJane Y Wu
Apr 18, 2009·Ophthalmic Genetics·Goranka Tanackovic, Carlo Rivolta
Jul 17, 2004·The EMBO Journal·Nina SchaffertReinhard Lührmann
Nov 5, 2013·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Liling WangLeqin Ke
Feb 25, 2003·Nature Structural Biology·Mohamed AittalebHong Li
Aug 17, 2021·Frontiers in Cell and Developmental Biology·Chunbo YangMajlinda Lako
Feb 26, 2003·Genes & Development·Nuno André Faustino, Thomas A Cooper

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