The PRRSV-Specific Memory B Cell Response Is Long-Lived in Blood and Is Boosted During Live Virus Re-exposure.

Frontiers in Immunology
Michael C RaheMichael P Murtaugh

Abstract

Porcine reproductive and respiratory syndrome virus (PRRSV) is an important pathogen of swine health and well-being worldwide largely due to an insufficient understanding of the adaptive immune response to infection leading to ineffective PRRSV control. The memory and anamnestic response to infection are critical gaps in knowledge in PRRSV immunity. The lack of effective tools for the evaluation of the memory response previously hindered the ability to effectively characterize the porcine memory response to infection. However, the creation and validation of a PRRSV nsp7-specific B cell tetramer now facilitates the ability to detect very rare memory B cells and thus define the memory response of the pig. Here, we describe the PRRSV nsp7-specific B cell response following vaccination and challenge in six key secondary lymphoid organs including the identification of PBMCs as the tissue of interest for the memory immune response in pigs. Following live virus challenge of immune animals, an anamnestic response of nsp7-specific memory B cells and neutralizing antibodies was observed. This characterization of the functional humoral immune response to PRRSV answers key questions involved in regional specialization of the immune respons...Continue Reading

References

Apr 17, 2004·Journal of Immunological Methods·Shane CrottyRafi Ahmed
Oct 19, 2007·Journal of Virology·Prasad MulupuriMichael P Murtaugh
Aug 31, 2010·Virus Research·Michael P MurtaughFrederick C-C Leung
Feb 7, 2012·Proceedings of the National Academy of Sciences of the United States of America·Taishi OnoderaKazuo Kobayashi
Feb 26, 2015·The Journal of General Virology·Manreetpal Singh BrarFrederick Chi-Ching Leung
Nov 20, 2016·European Journal of Immunology·Alaitz AranburuRita Carsetti
Feb 17, 2017·BMC Veterinary Research·Cheryl M T DvorakMichael P Murtaugh
Apr 18, 2017·Veterinary Immunology and Immunopathology·Michael C Rahe, Michael P Murtaugh
Aug 2, 2017·Viral Immunology·Michael C RaheMichael P Murtaugh
Aug 8, 2017·Science Immunology·LaTonya D WilliamsBarton F Haynes
Apr 27, 2018·Tropical Animal Health and Production·Chaitawat SirisereewanKomkrich Teankum

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Datasets Mentioned

BETA
AF066183.4
MN184
DQ176019.1
MN175677
AF159149.1

Methods Mentioned

BETA
ELISA
pulldown
pull-down
flow cytometry

Software Mentioned

GraphPad prism
GraphPad
BLASTn

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