The pseudo-receiver domain of CikA regulates the cyanobacterial circadian input pathway

Molecular Microbiology
Xiaofan ZhangSusan S Golden

Abstract

CikA (circadian input kinase) is a component of the cyanobacterial circadian clock that aids in synchronizing the endogenous oscillator with the external environment. cikA mutants of the prokaryotic circadian model organism Synechococcus elongatus PCC 7942 fail to reset the phase of the circadian rhythm of gene expression after an environmental time cue, and also exhibit reduced amplitude and shortened period of circadian oscillation. CikA has histidine protein kinase (HPK) activity that is modulated in vitro by GAF and pseudo-receiver (PsR) domains. Here we show that the PsR domain negatively regulates HPK activity in vivo and also serves as an interaction module to dock CikA at a specific subcellular location. Phenotypes conferred by alleles that encode CikA variants showed that all domains except the featureless N-terminus are required for CikA function. Overexpression of all alleles that encode the PsR domain, whether or not the HPK is functional, caused a dominant arrhythmic phenotype, whereas overexpressed variants that lack PsR did not. Subcellular localization of intact CikA identified a polar focus whereas a variant without PsR showed uniform distribution in the cell, consistent with a model in which PsR mediates inter...Continue Reading

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Citations

Feb 6, 2010·Proceedings of the National Academy of Sciences of the United States of America·Yasuhito TaniguchiTokitaka Oyama
Mar 17, 2010·Proceedings of the National Academy of Sciences of the United States of America·Thammajun L WoodSusan S Golden
Apr 19, 2008·Cold Spring Harbor Symposia on Quantitative Biology·S S Golden
Aug 11, 2012·Bioscience, Biotechnology, and Biochemistry·Hiroaki KatoHirofumi Yoshikawa
Nov 8, 2006·Proceedings of the National Academy of Sciences of the United States of America·Natalia B IvlevaSusan S Golden
Aug 25, 2012·Trends in Biochemical Sciences·Gerben van Ooijen, Andrew J Millar
Nov 6, 2008·Current Opinion in Microbiology·Guogang Dong, Susan S Golden
Nov 5, 2014·Photosynthesis Research·Rayka YokooDavid F Savage
Aug 16, 2014·Current Biology : CB·Susan E CohenSusan S Golden
Sep 13, 2008·Current Biology : CB·Carl Hirschie JohnsonYao Xu
Jan 7, 2014·Marine Genomics·Ilka M AxmannAnnegret Wilde
Aug 7, 2013·Proceedings of the National Academy of Sciences of the United States of America·Joseph S BoydSusan S Golden
Sep 4, 2015·Microbiology and Molecular Biology Reviews : MMBR·Susan E Cohen, Susan S Golden
Feb 14, 2019·Journal of Biological Rhythms·Manpreet KaurYong-Ick Kim
Jul 21, 2007·The Journal of Biological Chemistry·Ralf PaulUrs Jenal
Aug 1, 2018·Proceedings of the National Academy of Sciences of the United States of America·Susan E CohenSusan S Golden
Oct 11, 2008·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Rei NarikawaMasahiko Ikeuchi
Jan 17, 2020·Journal of Molecular Biology·Carrie L Partch

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