The Pseudomonas aeruginosa initiation factor IF-2 is responsible for formylation-independent protein initiation in P. aeruginosa.

The Journal of Biological Chemistry
Marta Steiner-MosonyiD Mangroo

Abstract

Formylation of the initiator methionyl-tRNA (Met-tRNAfMet) was generally thought to be essential for initiation of protein synthesis in all eubacteria based on studies conducted primarily in Escherichia coli. However, this view of eubacterial protein initiation has changed because some bacteria have been demonstrated to have the capacity to initiate protein synthesis with the unformylated Met-tRNAfMet. Here we show that the Pseudomonas aeruginosa initiation factor IF-2 is required for formylation-independent protein initiation in P. aeruginosa, the first bacterium shown to have the ability to initiate protein synthesis with both the initiator formyl-methionyl-tRNA (fMet-tRNAfMet) and Met-tRNAfMet. The E. coli IF-2, which participates exclusively in formylation-dependent protein initiation in E. coli, was unable to facilitate utilization of Met-tRNAfMet in initiation in P. aeruginosa. However, the E. coli IF-2 was made to function in formylation-independent protein initiation in P. aeruginosa by decreasing the positive charge potential of the cleft that binds the amino end of the amino acid attached to the tRNA. Furthermore increasing the positive charge potential of this cleft in the P. aeruginosa IF-2 prevented the protein fro...Continue Reading

References

Jul 20, 1991·Journal of Molecular Biology·S LaalamiM Grunberg-Manago
Dec 1, 1989·Genes & Development·D HartzL Gold
Jul 31, 1999·The Journal of Biological Chemistry·D T NewtonD Mangroo
Jun 22, 2000·Journal of Molecular Biology·L A KelleyM J Sternberg
Oct 3, 2000·The EMBO Journal·M GuenneuguesC O Gualerzi
Aug 15, 2001·Antimicrobial Agents and Chemotherapy·P MargolisJ Trias
Jan 24, 2002·Proceedings of the National Academy of Sciences of the United States of America·Brian J AkerleyJohn J Mekalanos
Apr 17, 2003·Proceedings of the National Academy of Sciences of the United States of America·K KobayashiN Ogasawara
May 6, 2003·Archives of Biochemistry and Biophysics·Cristiana GarofaloBoyd Hardesty
Jun 12, 2003·The Journal of Biological Chemistry·Anne S TibbettsDean R Appling

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Citations

Jan 28, 2009·Antimicrobial Agents and Chemotherapy·Yann DurocThierry Meinnel
Jun 25, 2008·Infection and Immunity·Kevin DybvigAnn E Loraine
Feb 6, 2010·Molecular Microbiology·Anna ZorzetDan I Andersson
Jun 7, 2005·Archives of Biochemistry and Biophysics·Cristiana GarofaloDean R Appling
Feb 26, 2005·The Journal of Biological Chemistry·Fabien CharrièreAndré Schneider
Nov 18, 2020·The Journal of Biological Chemistry·Sunil Shetty, Umesh Varshney
Jan 19, 2021·The Journal of Biological Chemistry·Sunil Shetty, Umesh Varshney
Dec 1, 2011·EcoSal Plus·Yves MechulamEmmanuelle Schmitt

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