The Pseudomonas aeruginosa Orphan Quorum Sensing Signal Receptor QscR Regulates Global Quorum Sensing Gene Expression by Activating a Single Linked Operon

MBio
Fengming DingAjai A Dandekar

Abstract

Pseudomonas aeruginosa uses two acyl-homoserine lactone signals and two quorum sensing (QS) transcription factors, LasR and RhlR, to activate dozens of genes. LasR responds to N-3-oxo-dodecanoyl-homoserine lactone (3OC12-HSL) and RhlR to N-butanoyl-homoserine lactone (C4-HSL). There is a third P. aeruginosa acyl-homoserine-lactone-responsive transcription factor, QscR, which acts to dampen or delay activation of genes by LasR and RhlR by an unknown mechanism. To better understand the role of QscR in P. aeruginosa QS, we performed a chromatin immunoprecipitation analysis, which showed this transcription factor bound the promoter of only a single operon of three genes linked to qscR, PA1895 to PA1897. Other genes that appear to be regulated by QscR in transcriptome studies were not direct targets of QscR. Deletion of PA1897 recapitulates the early QS activation phenotype of a QscR-null mutant, and the phenotype of a QscR-null mutant was complemented by PA1895-1897 but not by PA1897 alone. We conclude that QscR acts to modulate quorum sensing through regulation of a single operon, apparently raising the QS threshold of the population and providing a "brake" on QS autoinduction.IMPORTANCE Quorum sensing, a cell-cell communication s...Continue Reading

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Citations

Nov 9, 2018·World Journal of Microbiology & Biotechnology·Tapas DasUtpal Ch De
Feb 21, 2019·Journal of Bacteriology·Hui ZhouFeng Xu
Jul 2, 2019·Aesthetic Surgery Journal·Jennifer N WalkerTerence M Myckatyn
Mar 10, 2019·Proceedings of the National Academy of Sciences of the United States of America·Maxim KostylevAjai A Dandekar
Mar 15, 2020·Applied and Environmental Microbiology·Yanpeng YangChao Shi
Nov 3, 2020·Expert Review of Anti-infective Therapy·Mainul HaqueDilshad Jahan
Dec 31, 2020·ACS Omega·Mohd Farhan KhanIqbal Ahmad
May 11, 2021·Frontiers in Microbiology·Manoj KumarNobuhisa Masuda
Apr 11, 2021·Applied and Environmental Microbiology·Rhea G AbisadoJosephine R Chandler

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Datasets Mentioned

BETA
SRP149739
PRJNA472067

Methods Mentioned

BETA
immunoprecipitation
immunoprecipitation assay
ChIP-seq
electrophoretic mobility
PCR
electrophoresis
affinity purification

Software Mentioned

MACS
Regulatory Sequence Analysis Tools ( RSAT

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