The PTPase YopH inhibits uptake of Yersinia, tyrosine phosphorylation of p130Cas and FAK, and the associated accumulation of these proteins in peripheral focal adhesions

The EMBO Journal
C PerssonM Fällman

Abstract

Pathogenic Yersinia resist uptake by eukaryotic cells by a mechanism involving the virulence protein YopH, a protein tyrosine phosphatase. We show that p130Cas and FAK are phosphorylated and recruited to peripheral focal complexes during bacterial uptake in HeLa cells. The inactive form of YopH interacts with the tyrosine phosphorylated forms of FAK and p130Cas and co-localizes with these proteins in focal adhesions. On the other hand, the presence of active YopH results in inhibition of uptake, dephosphorylation of p130Cas and FAK, and disruption of peripheral focal complexes. We suggest that p130Cas and FAK are substrates for YopH and that the dephosphorylation of these proteins impairs the uptake of Yersinia pseudotuberculosis into HeLa cells.

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Citations

Aug 26, 1998·Folia Microbiologica·M FällmanH Wolf-Watz
Nov 26, 2009·Cellular and Molecular Life Sciences : CMLS·Nadezhda TikhmyanovaErica A Golemis
Mar 27, 2004·Microbial Pathogenesis·Rachid BenabdillahGuy R Cornelis
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