The pupal specifier broad directs progressive morphogenesis in a direct-developing insect.

Proceedings of the National Academy of Sciences of the United States of America
Deniz F ErezyilmazJames W Truman

Abstract

A key regulatory gene in metamorphosing (holometabolous) insect life histories is the transcription factor broad (br), which specifies pupal development. To determine the role of br in a direct-developing (hemimetabolous) insect that lacks a pupal stage, we cloned br from the milkweed bug, Oncopeltus fasciatus (Of'br). We find that, unlike metamorphosing insects, in which br expression is restricted to the larval-pupal transition, Of'br mRNA is expressed during embryonic development and is maintained at each nymphal molt but then disappears at the molt to the adult. Induction of a supernumerary nymphal stage with a juvenile hormone (JH) mimic prevented the disappearance of br mRNA. In contrast, induction of a precocious adult molt by application of precocene II to third-stage nymphs caused a loss of br mRNA at the precocious adult molt. Thus, JH is necessary to maintain br expression during the nymphal stages. Injection of Of'br dsRNA into either early third- or fourth-stage nymphs caused a repetition of stage-specific pigmentation patterns and prevented the normal anisometric growth of the wing pads without affecting isometric growth or molting. Therefore, br is necessary for the mutable (heteromorphic) changes that occur duri...Continue Reading

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Citations

May 29, 2007·Development Genes and Evolution·Rebecca F Spokony, Linda L Restifo
Feb 4, 2010·Development Genes and Evolution·Deniz F ErezyilmazLynn M Riddiford
Sep 22, 2012·Annual Review of Entomology·Marek JindraLynn M Riddiford
Apr 30, 2014·Proceedings of the National Academy of Sciences of the United States of America·Enric UreñaDavid Martín
Jul 8, 2009·Insect Biochemistry and Molecular Biology·Hua-Bing WangHideki Kawasaki
Jul 14, 2007·Insect Biochemistry and Molecular Biology·James W Truman, Lynn M Riddiford
Sep 26, 2008·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Josefa CruzXavier Bellés
Nov 2, 2014·Ecology and Evolution·Emily R Burdfield-Steel, David M Shuker
Jun 7, 2014·Wiley Interdisciplinary Reviews. Developmental Biology·H Frederik NijhoutViviane Callier
Jul 29, 2009·Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution·Ying Dong, Markus Friedrich
Jun 12, 2013·Insect Biochemistry and Molecular Biology·Jun-Bo LuanHenryk Czosnek
Nov 27, 2007·Mechanisms of Development·Chieka MinakuchiLynn M Riddiford
Feb 20, 2016·PloS One·Isabelle Mifom VeaChieka Minakuchi
Aug 24, 2019·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Yoshiyasu IshimaruTaro Mito
Aug 24, 2019·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Marek Jindra
Aug 24, 2019·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·James W Truman, Lynn M Riddiford

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