PMID: 1213685Dec 1, 1975Paper

The purification and amino acid sequence of toxin CM-13b from Naja haje annulifera (Egyptian cobra) venom

Hoppe-Seyler's Zeitschrift für physiologische Chemie
F J Joubert

Abstract

Toxin CM-13b was purified from the venom of Naja haje annulifera by gel filtration on Sephadex G-50 and by ion-exchange chromatography on CM-cellulose. The toxin comprises 65 amino acid residues and is cross-linked by five disulphide bridges. The complete amino acid sequence of toxin CM-13b was elucidated. The reduced and S-carboxymethylated toxin was digested with trypsin and chymotrypsin and the peptides purified by DEAE-cellulose chromatography and chromatography or electrophoresis on paper. The amino acid sequences of the intact toxin and its constituent peptides were determined by the Edman-Begg procedure, either through the use of the automatic sequenator or by manual manipulation. The chymotryptic digest provided the necessary overlapping peptides for aligning the tryptic peptides. The primary structure of toxin CM-13b shows a high degree of homology with that of protein S4C11 from Naja melanoleuca venom[1], but their toxicities are very different.

Citations

Jan 1, 1985·The International Journal of Biochemistry·F J Joubert
Jan 1, 1977·Toxicon : Official Journal of the International Society on Toxinology·Y IshikawaN Tamiya
Jan 1, 1980·Toxicon : Official Journal of the International Society on Toxinology·F J Joubert, N Taljaard
Jan 1, 1980·Toxicon : Official Journal of the International Society on Toxinology·F J Joubert, N Taljaard
Jan 1, 1982·Toxicon : Official Journal of the International Society on Toxinology·A L HarveyE Karlsson
Feb 27, 2001·Toxicon : Official Journal of the International Society on Toxinology·Y N UtkinV I Tsetlin
Mar 27, 2003·Toxicon : Official Journal of the International Society on Toxinology·S NirthananR M Kini
Jul 1, 1984·British Journal of Pharmacology·A L HarveyF J Joubert
May 1, 1980·Hoppe-Seyler's Zeitschrift für physiologische Chemie·F J Joubert, N Taljaard
Sep 1, 1982·Hoppe-Seyler's Zeitschrift für physiologische Chemie·F J JoubertN Taljaard
Apr 1, 1979·Hoppe-Seyler's Zeitschrift für physiologische Chemie·F J Joubert, N Taljaard
May 1, 1981·Hoppe-Seyler's Zeitschrift für physiologische Chemie·F J JoubertT Haylett
Dec 1, 1980·Hoppe-Seyler's Zeitschrift für physiologische Chemie·F J Joubert, D J Strydom
Jan 19, 2019·PLoS Neglected Tropical Diseases·Felipe Silva-de-FrançaDenise V Tambourgi

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.