The purification and characterization of ATP synthase complexes from the mitochondria of four fungal species

The Biochemical Journal
Sidong LiuJ E Walker

Abstract

The ATP synthases have been isolated by affinity chromatography from the mitochondria of the fungal species Yarrowia lipolytica, Pichia pastoris, Pichia angusta and Saccharomyces cerevisiae. The subunit compositions of the purified enzyme complexes depended on the detergent used to solubilize and purify the complex, and the presence or absence of exogenous phospholipids. All four enzymes purified in the presence of n-dodecyl-β-D-maltoside had a complete complement of core subunits involved directly in the synthesis of ATP, but they were deficient to different extents in their supernumerary membrane subunits. In contrast, the enzymes from P. angusta and S. cerevisiae purified in the presence of n-decyl-β-maltose neopentyl glycol and the phospholipids 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine, 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine, cardiolipin (diphosphatidylglycerol) and 1-palmitoyl-2-oleoyl-sn-glycero-3-[phospho-rac-(1-glycerol)] had a complete complement of core subunits and also contained all of the known supernumerary membrane subunits, e, f, g, j, k and ATP8 (or Aap1), plus an additional new membrane component named subunit l, related in sequence to subunit k. The catalytic domain of the enzyme from P...Continue Reading

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Citations

Feb 14, 2016·Biochimica Et Biophysica Acta·Miriam Vázquez-AcevedoDiego González-Halphen
Dec 24, 2015·Frontiers in Plant Science·Marco ZancaniAngelo Vianello
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Oct 28, 2017·Science·Hui GuoJohn L Rubinstein
Apr 9, 2015·The Journal of Biological Chemistry·Jennifer LeeJohn E Walker
Oct 13, 2015·Acta Crystallographica. Section F, Structural Biology Communications·Edgar Morales-RíosJohn E Walker
Nov 27, 2018·Biochemistry. Biokhimii︠a︡·A S Lapashina, B A Feniouk

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Methods Mentioned

BETA
X-ray
reverse-phase chromatography
electrophoresis
PCR

Software Mentioned

Mascot
tBLASTn

Related Concepts

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ATP Synthases

ATP synthases are enzymes located in the inner mitochondrial membrane that catalyze the synthesis of ATP during cellular respiration. Discover the latest research on ATP synthases here.