The purification and partial characterization of an insulin-like protein from human serum

The Journal of Clinical Investigation
P L Poffenbarger

Abstract

A preparative scheme has been developed for the purification of a trace protein in human serum exhibiting nonsuppressible insulin-like activity (NSILA). This scheme consisted of (a) adsorption chromatography of serum utilizing the sulfonic acid polystyrene resin, Dowex 50, at pH 6.8; (b) (200 gel filtration at pH 8.9; and (c) acrylamide gel electrophoresis in a discontinuous preparative system. Throughout all procedures, NSILA fractionated as a single molecular species approximating 90,000 mol wt. The purified protein exhibited a single band by disk gel electrophoresis, an isoelectric pH approximating 6.2, doublet bands of 90,000 mol mt by analytical sodium dodecyl sulfate gel electrophoresis, and a biologic specific activity approximating 50 mU/mg. Serum somatomedin (sulfation factor) activtiy did not fractionate with NSILA in this scheme, and partially purified NSILA did not stimulate radiosulfate uptake into hypophysectomized rat costal cartilage. This protein appears to represent the major constituent of serum NSILA: its purification and partial characterization provides the first step towards elucidation of its metabolic role.

References

May 1, 1974·The Journal of Clinical Endocrinology and Metabolism·K MegyesiP Gorden
Jul 1, 1970·Metabolism: Clinical and Experimental·P L PoffenbargerJ Steinke
Jan 1, 1957·Scandinavian Journal of Clinical and Laboratory Investigation·T ARONSSON, A GRONWALL

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Citations

Jan 1, 1992·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·G H TeschA C Herington
Oct 1, 1976·Diabetologia·R M El-AllawyK Johansen
Mar 1, 1981·Biological Trace Element Research·H P Roth, M Kirchgessner
Jan 1, 1983·The International Journal of Biochemistry·H J Cornell, A C Herington
Jan 1, 1983·The International Journal of Biochemistry·A C HeringtonA D Kuffer
Jan 1, 1985·The International Journal of Biochemistry·H J CornellA C Herington
Nov 1, 1979·Molecular and Cellular Endocrinology·R C FranklinA C Herington
Feb 14, 1980·The New England Journal of Medicine·L S Phillips, R Vassilopoulou-Sellin
Dec 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·D J Knauer, G L Smith
Apr 1, 1976·The Journal of Clinical Investigation·G BurghenP L Poffenbarger
Sep 1, 2011·Mass Spectrometry Reviews·Peihong ZhuJohn G Marshall
Jan 1, 1984·Metabolism: Clinical and Experimental·C A StuartR W Furlanetto
Mar 1, 1984·Metabolism: Clinical and Experimental·M A KhokherP Dandona
Jun 1, 1988·Diabetes/metabolism Reviews·M Press
Jan 1, 1976·Comparative Biochemistry and Physiology. A, Comparative Physiology·P L PoffenbargerA Bennett-Novak
Mar 7, 2009·Journal of Proteome Research·Monika TucholskaJohn Marshall
Jan 1, 1979·The American Journal of Medicine·H PlovnickP L Poffenbarger
Sep 29, 1978·Biochemical and Biophysical Research Communications·S B Leichter, P L Poffenbarger
Jul 1, 1980·Clinics in Endocrinology and Metabolism·C R Kahn
Jan 29, 2011·Colloids and Surfaces. B, Biointerfaces·Müfrettin Murat SarıAdil Denizli

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