PMID: 4990583Jun 1, 1970

The purification and properties of a ribonucleoenzyme, o-diphenol oxidase, from potatoes

The Biochemical Journal
K Balasingam, W Ferdinand

Abstract

1. o-Diphenol oxidase was isolated from potato tubers by a new approach that avoids the browning due to autoxidation. 2. There are at least three forms of the enzyme, of different molecular weights. The major form, of highest molecular weight, was separated from the others in good yield and with high specific activity by gel filtration through Bio-Gel P-300. 3. The major form is homogeneous by disc electrophoresis but regenerates small amounts of the species of lower molecular weight, as shown by rechromatography on Bio-Gel P-300. 4. There is an equal amount of RNA and protein by weight in the fully active enzyme. The RNA cannot be removed without loss of activity, and is not attacked by ribonuclease. 5. The pH optimum of the enzyme is at pH5.0 when assayed with 4-methylcatechol as substrate. It is ten times more active with this substrate than with chlorogenic acid or catechol. The enzyme is fully active in 4m-urea. 6. A minimal molecular weight of 36000 is indicated by copper content and amino acid analysis of the protein component of the enzyme. 7. The protein contains five half-cystinyl residues per 36000 daltons, a value similar to that found in o-diphenol oxidase from mushrooms. It also contains tyrosine residues although...Continue Reading

Citations

Nov 12, 1990·FEBS Letters·N K TikhomirovaG A Kochetov
May 1, 1975·European Journal of Biochemistry·S Gutteridge, D Robb
Sep 1, 1984·Bioscience Reports·R W Davies
Feb 13, 1986·Nature·F H Westheimer
May 15, 1985·Archives of Biochemistry and Biophysics·J D Weinstein, S I Beale
Jan 1, 1980·Folia Microbiologica·M SvábováL Marvanová
May 15, 1979·European Journal of Biochemistry·G A KorneevaA A Bayev
Feb 1, 1986·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·D S Waugh, N R Pace
Dec 18, 1972·European Journal of Biochemistry·K Lerch, L Ettinger
Jan 1, 1985·Origins of Life and Evolution of the Biosphere : the Journal of the International Society for the Study of the Origin of Life·N R Pace, T L Marsh
Apr 7, 1975·Zeitschrift Für Lebensmittel-Untersuchung Und -Forschung·G Matheis, H D Belitz
Sep 19, 1973·Nature: New Biology·V J Hearing
Jan 1, 1974·International Journal of Peptide and Protein Research·T G HoyP M Harrison

Related Concepts

Molecular Sieve Chromatography
Copper
Catechol Oxidase
Electrophoresis, Disc
Hydrogen-Ion Concentration
Gene Products, Protein
RNA

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Synthetic Genetic Array Analysis

Synthetic genetic arrays allow the systematic examination of genetic interactions. Here is the latest research focusing on synthetic genetic arrays and their analyses.

Neural Activity: Imaging

Imaging of neural activity in vivo has developed rapidly recently with the advancement of fluorescence microscopy, including new applications using miniaturized microscopes (miniscopes). This feed follows the progress in this growing field.

Computational Methods for Protein Structures

Computational methods employing machine learning algorithms are powerful tools that can be used to predict the effect of mutations on protein structure. This is important in neurodegenerative disorders, where some mutations can cause the formation of toxic protein aggregations. This feed follows the latests insights into the relationships between mutation and protein structure leading to better understanding of disease.

Congenital Hyperinsulinism

Congenital hyperinsulinism is caused by genetic mutations resulting in excess insulin secretion from beta cells of the pancreas. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Epigenetic Memory

Epigenetic memory refers to the heritable genetic changes that are not explained by the DNA sequence. Find the latest research on epigenetic memory here.

Cell Atlas of the Human Eye

Constructing a cell atlas of the human eye will require transcriptomic and histologic analysis over the lifespan. This understanding will aid in the study of development and disease. Find the latest research pertaining to the Cell Atlas of the Human Eye here.

Femoral Neoplasms

Femoral Neoplasms are bone tumors that arise in the femur. Discover the latest research on femoral neoplasms here.