PMID: 39606Jun 6, 1979

The purification of glutamine synthetase from Azotobacter and other procaryotes by blue sepharose chromatography

Biochimica Et Biophysica Acta
J E LepoF R Tabita

Abstract

We report the facile purification of glutamine synthetase (L-glutamate: ammonia ligase (adenosine 5'-diphosphate-forming), EC 6.3.1.2) in both the adenylylated and unadenylylated form, from Azotobacter vinelandii ATCC 12837. A general affinity column, which used as an affinity ligand Reactive blue 2 dye (Cibacron blue) covalently linked to Agarose, was employed as an efficient first step of purification. Further purification to electrophoretic homogeneity employed DEAE-cellulose chromatography and an additional Affigel chromatographic step. The method was used successfully to prepare glutamine synthetase from Escherichia coli, Rhodopseudomonas sphaeroides and Anabaena sp. strain CA.

References

Sep 1, 1980·European Journal of Biochemistry·D PomponF Lederer
Jan 1, 1981·Archives of Biochemistry and Biophysics·R Tuli, J Thomas
Mar 31, 2006·Journal of Enzyme Inhibition and Medicinal Chemistry·S Sumathi, Dipak Dasgupta

Citations

Feb 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·S G Rhee, P B Chock
Feb 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·S T ThompsonE Stellwagen
Feb 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·S T Thompson, E Stellwagen
Aug 22, 1977·Biochemical and Biophysical Research Communications·S P SchenkO Wyss
Jul 1, 1974·Archives of Biochemistry and Biophysics·R E MillerE R Stadtman
Sep 26, 1966·Archives of Biochemistry and Biophysics·C A WoolfolkE R Stadtman
Sep 24, 2009·Therapeutic Advances in Cardiovascular Disease·Erkki PesonenPirkko J Pussinen

Related Concepts

Alkalescens-Dispar Group
Adenosine
GLUL
SDS-PAGE
Glutamate-Ammonia Ligase
Chromatography, DEAE-Cellulose
GLUL gene
Chromatography, Agarose
Blood Ammonia Measurement
Ammonia Inhalants

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