PMID: 39606Jun 6, 1979

The purification of glutamine synthetase from Azotobacter and other procaryotes by blue sepharose chromatography

Biochimica Et Biophysica Acta
J E LepoF R Tabita


We report the facile purification of glutamine synthetase (L-glutamate: ammonia ligase (adenosine 5'-diphosphate-forming), EC in both the adenylylated and unadenylylated form, from Azotobacter vinelandii ATCC 12837. A general affinity column, which used as an affinity ligand Reactive blue 2 dye (Cibacron blue) covalently linked to Agarose, was employed as an efficient first step of purification. Further purification to electrophoretic homogeneity employed DEAE-cellulose chromatography and an additional Affigel chromatographic step. The method was used successfully to prepare glutamine synthetase from Escherichia coli, Rhodopseudomonas sphaeroides and Anabaena sp. strain CA.


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Feb 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·S G Rhee, P B Chock
Feb 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·S T ThompsonE Stellwagen
Feb 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·S T Thompson, E Stellwagen
Aug 22, 1977·Biochemical and Biophysical Research Communications·S P SchenkO Wyss
Jul 1, 1974·Archives of Biochemistry and Biophysics·R E MillerE R Stadtman
Sep 26, 1966·Archives of Biochemistry and Biophysics·C A WoolfolkE R Stadtman
Sep 24, 2009·Therapeutic Advances in Cardiovascular Disease·Erkki PesonenPirkko J Pussinen

Related Concepts

Alkalescens-Dispar Group
Glutamate-Ammonia Ligase
Chromatography, DEAE-Cellulose
GLUL gene
Chromatography, Agarose
Blood Ammonia Measurement
Ammonia Inhalants

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