PMID: 8962071Dec 10, 1996Paper

The purified Bacillus subtilis tetracycline efflux protein TetA(L) reconstitutes both tetracycline-cobalt/H+ and Na+(K+)/H+ exchange

Proceedings of the National Academy of Sciences of the United States of America
J ChengT A Krulwich

Abstract

Recent work has suggested that the chromosomally encoded TetA(L) transporter of Bacillus subtilis, for which no physiological function had been shown earlier, not only confers resistance to low concentrations of tetracycline but is also a multifunctional antiporter protein that has dominant roles in both Na(+)- and K(+)-dependent pH homeostasis and in Na+ resistance during growth at alkaline pH. To rigorously test this hypothesis, TetA(L) has been purified with a hexahistidine tag at its C terminus and reconstituted into proteoliposomes. The TetA(L)-hexahistidine proteoliposomes exhibit high activities of tetracycline-cobalt/H+, Na+/ H+, and K+/H+ antiport in an assay in which an outwardly directed proton gradient is artificially imposed and solute uptake is monitored. Tetracycline uptake depends on the presence of cobalt and vice versa, with the cosubstrates being transported in a 1:1 ratio. Evidence for the electrogenicity of both tetracycline-cobalt/H+ and Na+/H+ antiports is presented. K+ and Li+ inhibit Na+ uptake, but there is little cross-inhibition between Na+ and tetracycline-cobalt uptake activities. The results strongly support the conclusion that TetA(L) is a multifunctional antiporter. They expand the roster of suc...Continue Reading

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Citations

Jul 12, 2002·FEMS Immunology and Medical Microbiology·James BurnieRuth Matthews
Jun 15, 2005·Nature Reviews. Microbiology·Terry A KrulwichEitan Bibi
Feb 13, 1999·Molecular Microbiology·S J StasinopoulosD H Bechhofer
May 14, 2004·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Gilliane GuillaumeJean-Marc Collard
Jul 25, 2012·Genome Research·Tim van Opijnen, Andrew Camilli
Dec 21, 2004·Protein Science : a Publication of the Protein Society·Alla KorepanovaTimothy A Cross
Oct 4, 2006·Journal of Bacteriology·Yi WeiDavid H Bechhofer
Nov 28, 2000·Journal of Bacteriology·S GertzM Hecker
Nov 10, 2005·Biochimica Et Biophysica Acta·Etana PadanTerry A Krulwich
Jul 17, 2001·Molecular Microbiology·T WiegertW Schumann
Jul 25, 2019·World Journal of Microbiology & Biotechnology·Kaixiang ShiGejiao Wang
Mar 25, 2021·Chemical Reviews·Peter J F HendersonKarl A Hassan
Feb 15, 2003·Biochimica Et Biophysica Acta·Da-Neng WangXiao-Dan Li
Jun 1, 1997·Journal of Bacteriology·M ItoT A Krulwich
Jul 8, 2021·International Journal of Systematic and Evolutionary Microbiology·Ya-Lin YinLei Wang

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