The putative Arabidopsis homolog of yeast vps52p is required for pollen tube elongation, localizes to Golgi, and might be involved in vesicle trafficking

Plant Physiology
Eglantine LobsteinS Bonhomme

Abstract

The screening of the Versailles collection of Arabidopsis T-DNA transformants allowed us to identify several male gametophytic mutants, including poky pollen tube (pok). The pok mutant, which could only be isolated as a hemizygous line, exhibits very short pollen tubes, explaining the male-specific transmission defect observed in this line. We show that the POK gene is duplicated in the Arabidopsis genome and that the predicted POK protein sequence is highly conserved from lower to higher eukaryotes. The putative POK homolog in yeast (Saccharomyces cerevisiae), referred to as Vps52p/SAC2, has been shown to be located at the late Golgi and to function in a complex with other proteins, Vps53p, Vps54p, and Vps51p. This complex is involved in retrograde trafficking of vesicles between the early endosomal compartment and the trans-Golgi network. We present the expression patterns of the POK gene and its duplicate P2 in Arabidopsis, and of the putative Arabidopsis homologs of VPS53 and VPS54 of yeast. We show that a POK::GFP fusion protein localizes to Golgi in plant cells, supporting the possibility of a conserved function for Vps52p and POK proteins. These results, together with the expression pattern of the POK::GUS fusion and the...Continue Reading

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Citations

Apr 19, 2011·Molecules and Cells·Min-Jung HanGynheung An
Jun 28, 2008·Journal of Experimental Botany·Hélène GuermonprezSandrine Bonhomme
Jan 30, 2007·Plant Physiology·Yuki FujikiYoshinori Ohsumi
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Sep 23, 2006·Plant & Cell Physiology·Min-Jung HanGynheung An
Apr 24, 2020·Proceedings of the National Academy of Sciences of the United States of America·María Otilia DelgadilloEnrique Rojo
May 28, 2019·Frontiers in Plant Science·Hyo-Young ParkJeong-Kook Kim
Mar 3, 2021·The Plant Journal : for Cell and Molecular Biology·Reneé RobinsonLaurian S Robert

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