The putative G protein-coupled receptor GrlD mediates extracellular polyphosphate sensing in Dictyostelium discoideum

Molecular Biology of the Cell
Patrick M SuessRichard H Gomer

Abstract

Five or more orthophosphates bound together by high-energy phosphoanhydride bonds are highly ubiquitous inorganic molecules called polyphosphate. Polyphosphate acts as a signaling molecule eliciting a number of responses in eukaryotic cells, but the mechanisms mediating these effects are poorly understood. Proliferating Dictyostelium discoideum cells accumulate extracellular polyphosphate. At extracellular concentrations similar to those observed in stationary phase cells, polyphosphate inhibits proteasome activity and proliferation, and induces aggregation. Here we identify GrlD as a putative G protein-coupled receptor that mediates binding of extracellular polyphosphate to the cell surface. Cells lacking GrlD do not respond to polyphosphate-induced proteasome inhibition, aggregation, or proliferation inhibition. Polyphosphate also elicits differential effects on cell-substratum adhesion and cytoskeletal F-actin levels based on nutrient availability, and these effects were also mediated by GrlD. Starving cells also accumulate extracellular polyphosphate. Starved cells treated with exopolyphosphatase failed to aggregate effectively, suggesting that polyphosphate also acts as a signaling molecule during starvation-induced develo...Continue Reading

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Citations

Jan 25, 2020·BioTechniques·Yu Tang, Richard H Gomer
Aug 19, 2020·Journal of Thrombosis and Haemostasis : JTH·Patrick M SuessJames H Morrissey
Jan 10, 2021·Current Genetics·Alix Denoncourt, Michael Downey
Dec 4, 2020·Proceedings of the National Academy of Sciences of the United States of America·Ramesh RijalRichard H Gomer
Mar 16, 2021·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Rahul GawriRita A Kandel

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Methods Mentioned

BETA
reverse transcription PCR
PCR
PCRs

Software Mentioned

ImageLab
Prism
QuantStudio
GraphPad

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