The putative protein methyltransferase LAE1 controls cellulase gene expression in Trichoderma reesei.

Molecular Microbiology
Bernhard SeibothChristian P Kubicek

Abstract

Trichoderma reesei is an industrial producer of enzymes that degrade lignocellulosic polysaccharides to soluble monomers, which can be fermented to biofuels. Here we show that the expression of genes for lignocellulose degradation are controlled by the orthologous T. reesei protein methyltransferase LAE1. In a lae1 deletion mutant we observed a complete loss of expression of all seven cellulases, auxiliary factors for cellulose degradation, β-glucosidases and xylanases were no longer expressed. Conversely, enhanced expression of lae1 resulted in significantly increased cellulase gene transcription. Lae1-modulated cellulase gene expression was dependent on the function of the general cellulase regulator XYR1, but also xyr1 expression was LAE1-dependent. LAE1 was also essential for conidiation of T. reesei. Chromatin immunoprecipitation followed by high-throughput sequencing ('ChIP-seq') showed that lae1 expression was not obviously correlated with H3K4 di- or trimethylation (indicative of active transcription) or H3K9 trimethylation (typical for heterochromatin regions) in CAZyme coding regions, suggesting that LAE1 does not affect CAZyme gene expression by directly modulating H3K4 or H3K9 methylation. Our data demonstrate that ...Continue Reading

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Citations

Mar 8, 2013·Journal of Industrial Microbiology & Biotechnology·Shaowen WangMiao Xing
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Datasets Mentioned

BETA
JN791097

Methods Mentioned

BETA
immunoprecipitation
PCR
ChIP-seq
ChIP
phosphotransferase

Software Mentioned

psi
blastp
blast
IQ5 Optical system
REST©
MEGA
tatistica
phi

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