The pyramid of transgenes TsVP and BetA effectively enhances the drought tolerance of maize plants

Plant Biotechnology Journal
Aiying WeiJuren Zhang

Abstract

Stress resistance has repeatedly been enhanced in plants by the transfer of a single gene using genetic engineering. However, further enhancement of resistance to abiotic stress is still necessary. In our research, maize plants that were transgenic for both betA (encoding choline dehydrogenase from Escherichia coli) and TsVP (encoding V-H+ -PPase from Thellungiella halophila) were produced by cross-pollination. The existence of the transgenes in the pyramided plants was demonstrated by PCR and Southern blotting. The stable expression of transgenes was confirmed by real-time RT-PCR (reverse transcription polymerase chain reaction) analysis. An examination of the drought resistance characteristics demonstrated that the pyramided transgenic plants had higher glycinebetaine contents and H+ -PPase activity compared with the parental lines, which had either betA or TsVP, and contained higher relative water content (RWC), greater solute accumulation and lower cell damage under drought stress treatment. The pyramided plants grew more vigorously with less growth retardation, shorter anthesis-silking interval and higher yields than their parental lines and the wild-type. We concluded that co-expression of the two genes involved in differ...Continue Reading

References

Apr 5, 2000·Biochimica Et Biophysica Acta·M Maeshima
Aug 12, 2000·Journal of Experimental Botany·K O HolmströmB Welin
Sep 27, 2001·Proceedings of the National Academy of Sciences of the United States of America·R A GaxiolaG R Fink
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Jun 18, 2003·Cell·Yigong Shi, Joan Massagué
Jul 1, 1954·The Biochemical Journal·E W YEMM, A J WILLIS
Oct 11, 2003·The Plant Journal : for Cell and Molecular Biology·Ronan SulpiceNorio Murata
Oct 8, 2005·Science·Jisheng LiRoberto Gaxiola
Dec 20, 2005·Proceedings of the National Academy of Sciences of the United States of America·Sunghun ParkRoberto A Gaxiola
Dec 7, 2006·Plant Biotechnology Journal·Ruidang QuanJuren Zhang
May 1, 2007·Current Opinion in Plant Biology·Motoaki SekiKazuo Shinozaki
Jul 25, 2007·Current Opinion in Plant Biology·Edward E Farmer, Céline Davoine
Oct 29, 1982·Science·J S Boyer
Nov 15, 2007·Plant Biotechnology Journal·Bei LiJuren Zhang
May 1, 2008·Annual Review of Plant Biology·Rana Munns, Mark Tester

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Citations

Jun 7, 2013·Transgenic Research·Matthias FladungM Raj Ahuja
Apr 3, 2012·Journal of Experimental Botany·Nicky J Atkinson, Peter E Urwin
Dec 10, 2013·Annual Review of Plant Biology·Honghong Hu, Lizhong Xiong
Dec 14, 2011·Current Opinion in Biotechnology·Jill DeikmanJacqueline E Heard
Oct 23, 2014·Cellular and Molecular Life Sciences : CMLS·Yujie Fang, Lizhong Xiong
Sep 18, 2015·F1000Research·Damar López-ArredondoLuis Herrera
Jul 15, 2018·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Xiang ZhuWubei Dong
Nov 13, 2020·Physiologia Plantarum·Anjali ShailaniAshwani Pareek
Jul 28, 2018·Biotechnology Advances·Caroline M Smith-Moore, Amy M Grunden

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