The QKI-6 RNA binding protein regulates actin-interacting protein-1 mRNA stability during oligodendrocyte differentiation.

Molecular Biology of the Cell
Evgueni DoukhanineStéphane Richard

Abstract

The quaking viable (qk(v)) mice represent an animal model of dysmyelination. The absence of expression of the QKI-6 and QKI-7 cytoplasmic isoforms in oligodendrocytes (OLs) during CNS myelination causes the qk(v) mouse phenotype. The QKI RNA-binding proteins are known to regulate RNA metabolism of cell cycle proteins and myelin components in OLs; however, little is known of their role in reorganizing the cytoskeleton or process outgrowth during OL maturation and differentiation. Here, we identify the actin-interacting protein (AIP)-1 mRNA as a target of QKI-6 by using two-dimensional differential gel electrophoresis. The AIP-1 mRNA contains a consensus QKI response element within its 3'-untranslated region that, when bound by QKI-6, decreases the half-life of the AIP-1 mRNA. Although the expression of QKI-6 is known to increase during OL differentiation and CNS myelination, we show that this increase is paralleled with a corresponding decrease in AIP-1 expression in rat brains. Furthermore, qk(v)/qk(v) mice that lack QKI-6 and QKI-7 within its OLs had an increased level of AIP-1 in OLs. Moreover, primary rat OL precursors harboring an AIP-1 small interfering RNA display defects in OL process outgrowth. Our findings suggest that...Continue Reading

References

Apr 6, 2001·Cellular and Molecular Life Sciences : CMLS·C M Brennan, J A Steitz
Mar 28, 2002·Proceedings of the National Academy of Sciences of the United States of America·Jiang I WuKaren Artzt
Dec 7, 2002·Neuron·Daniel LarocqueStéphane Richard
Nov 5, 2003·Journal of Neurobiology·Patrick D Sarmiere, James R Bamburg
May 4, 2005·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Jack D Keene, Patrick J Lager
Jul 22, 2005·Journal of Neuroscience Research·Sarah K WilliamsSusan C Barnett
Jul 26, 2005·Nature Structural & Molecular Biology·André Galarneau, Stéphane Richard
Jul 21, 2006·Molecular Biology of the Cell·Lixia ZhaoYue Feng
Nov 3, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Lixia ZhaoYue Feng
Feb 28, 2007·The Journal of Biological Chemistry·Shuxian JiangShalom Avraham
Sep 6, 2007·Journal of Neuroscience Research·Carol Anne Chénard, Stéphane Richard
Dec 1, 2007·WormBook : the Online Review of C. Elegans Biology·Min-Ho Lee, Tim Schedl
Jan 4, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·John D CahoyBen A Barres
Jan 15, 2008·Trends in Genetics : TIG·Talila VolkHila Toledano-Katchalski
Jan 23, 2008·Nature Reviews. Neuroscience·Chi W PakJames R Bamburg
Apr 30, 2008·Biochimica Et Biophysica Acta·John H CarsonElisa Barbarese
Jul 4, 2008·Trends in Genetics : TIG·Kiven E LukongStéphane Richard
Jul 30, 2008·The Journal of Cell Biology·Hao Yuan KuehWilliam M Brieher
May 21, 2009·Glia·Nina G BauerCharles Ffrench-Constant

❮ Previous
Next ❯

Citations

Jan 17, 2013·Human Molecular Genetics·Katarzyna J RadomskaElena Jazin
Jan 16, 2013·Molecular and Cellular Biology·Yunling WangStéphane Richard
Apr 12, 2014·PLoS Genetics·Feng-Yang ZongJingyi Hui
Dec 1, 2012·Scientific Reports·Salla RuskamoPetri Kursula
Feb 23, 2012·Scientific Reports·Christina Gavino, Stéphane Richard
Mar 19, 2016·Wiley Interdisciplinary Reviews. RNA·Lama Darbelli, Stéphane Richard
Jun 10, 2015·Biochemical and Biophysical Research Communications·Shuai WangJianhua Zhu
Nov 21, 2012·The International Journal of Neuropsychopharmacology·Yuan RenLan Xiao
Jan 5, 2016·PloS One·Katarzyna J RadomskaLina S Emilsson
Apr 30, 2016·The Journal of Biological Chemistry·Koichiro IrieKinichi Nakashima
Feb 12, 2017·The Journal of Biological Chemistry·Merlin P ThangarajAdil J Nazarali
Jun 24, 2018·Cancer Research·Hailong PeiTom K Hei
Jul 6, 2021·Frontiers in Cell and Developmental Biology·Xinyun ChenWeinian Shou

❮ Previous
Next ❯

Methods Mentioned

BETA
transgenic
electrophoresis
SELEX
transfection
Assay
in vitro transcription
PCR
electrophoretic mobility shift
coimmunoprecipitation
immunoprecipitations

Software Mentioned

AxioVision
Real
Scion
Image
pTKrenilla
Time PCR software

Related Concepts

Related Feeds

Brain developing: Influences & Outcomes

This feed focuses on influences that affect the developing brain including genetics, fetal development, prenatal care, and gene-environment interactions. Here is the latest research in this field.