PMID: 6403547Jan 1, 1983Paper

The quantitative immunochemical determination of pneumococcal and meningococcal capsular polysaccharides by light scattering rate nephelometry

Journal of Biological Standardization
C J Lee

Abstract

A quantitative nephelometric method was used for the measurement of the individual pneumococcal, as well as meningococcal, polysaccharides in the polyvalent vaccine final containers. This method is simple, rapid, inexpensive, and provides both qualitative and quantitative analyses of the polyvalent polysaccharide vaccines. By this method the individual pneumococcal types, 1, 2, 3, 4, 6A, 7F, 8, 9N, 12F, 14, 18C, 19F, 23F and 25 polysaccharides, were found to be present at 90-114% of the manufacturer's indicated concentrations; meningococcal group A, C, Y and W135 polysaccharides were at 90-108% of the manufacturer's listed concentrations. This nephelometric method coupled with gel filtration can also be used for measurement of the molecular sizes or stability of individual polysaccharides in the final container. Pneumococcal polysaccharide types 3, 6A, 9N and 19F, used as representative types, were treated with 0.5 N hydrochloric acid. The molecular sizes for types 3 and 9 N polysaccharides were stable to acid treatment. In contrast, types 6A and 19F polysaccharides were degraded. Heating meningococcal groups A, C, Y and W135 polysaccharides at 37 degrees C for 48 h did not affect their molecular size in the polyvalent vaccine.

References

Sep 1, 1977·Carbohydrate Research·B LindbergD A Powell
Nov 1, 1972·Carbohydrate Research·A S ChaudhariR J Fielder
Jul 14, 1973·Clinica Chimica Acta; International Journal of Clinical Chemistry·O J BjerrumB Weeke
Jan 1, 1980·Journal of Immunological Methods·I Deverill, W G Reeves

❮ Previous
Next ❯

Citations

Mar 5, 2005·Analytical Biochemistry·David H LambPatricia Cash
Jul 20, 2002·Biologicals : Journal of the International Association of Biological Standardization·Chi Jen Lee
Jan 1, 1991·Critical Reviews in Microbiology·C J LeeJ P Li
Jan 1, 1997·Critical Reviews in Microbiology·C J LeeS S Tai
Jan 16, 2018·Bioanalysis·Thorsten VerchMary Shank-Retzlaff
Mar 9, 2000·Analytical Biochemistry·C AbeygunawardanaJ P Hennessey

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Pneumonia (ASM)

Bacterial pneumonia is a prevalent and costly infection that is a significant cause of morbidity and mortality in patients of all ages. Here is the latest research.

Bacterial Pneumonia

Bacterial pneumonia is a prevalent and costly infection that is a significant cause of morbidity and mortality in patients of all ages. Here is the latest research.