The quantity of naturally processed peptides stably bound by HLA-A*0201 is significantly reduced in the absence of tapasin

Tissue Antigens
L D BarberTim Elliott

Abstract

Tapasin plays a critical role in promoting peptide binding by major histocompatibility complex (MHC) class I molecules in the endoplasmic reticulum. In its absence, cell surface expression of most allotypes is significantly reduced. Two exceptions are HLA-A*0201 and HLA-B*2705. In this study, the repertoire of peptides bound endogenously by these allotypes in the absence of tapasin was examined and stability of the HLA class I/peptide complexes assessed. Similar quantities of peptides were recovered from B*2705 complexes expressed in the absence and presence of tapasin and the composition of the peptide pools were not radically different. However, the stability of B*2705 molecules expressed at the surface of tapasin-deficient cells was found to be reduced which suggests there are subtle changes to the peptide repertoire. The impact of the absence of tapasin was more dramatic for A*0201. Although equivalent levels of cell surface A*0201 are expressed in the presence and absence of tapasin, very little A*0201 glycoprotein was recovered from tapasin-deficient cells suggesting the complexes readily dissociate. Consistent with reduced stability, A*0201 complexes were found to be rapidly lost from the surface of tapasin-deficient cel...Continue Reading

References

Sep 1, 1992·European Journal of Immunology·O RötzschkeH G Rammensee
Sep 26, 1991·Nature·T S JardetzkyD C Wiley
Aug 1, 1982·Human Immunology·S A EllisA McMichael
Dec 1, 1995·The Journal of Experimental Medicine·P M van EndertJ F Bach
Jan 13, 2000·Immunological Reviews·P CresswellG Diedrich
Jan 6, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·A W PurcellJ McCluskey
Mar 29, 2001·Trends in Immunology·A G Grandea, L Van Kaer

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Citations

Aug 30, 2008·Annual Review of Cell and Developmental Biology·David R Peaper, Peter Cresswell
Aug 3, 2004·Proceedings of the National Academy of Sciences of the United States of America·Mark HowarthTim Elliott
Dec 13, 2006·Developmental and Comparative Immunology·Sven Martin JørgensenTor Gjøen
Sep 27, 2005·Immunological Reviews·Tim Elliott, Anthony Williams
Jan 22, 2014·Immunology·Keith M PorterLouise H Boyle
Jan 6, 2009·BMC Bioinformatics·Hong Huang LinVladimir Brusic
May 16, 2019·Critical Reviews in Biochemistry and Molecular Biology·Kannan NatarajanDavid H Margulies
Jun 10, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Laura SesmaJosé A López de Castro
Oct 29, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Clemens HermannLouise H Boyle
Nov 21, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Maya W Everett, Michael Edidin
Aug 28, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Linda GeironsonKajsa M Paulsson
Nov 11, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Angela L ZarlingVictor H Engelhard

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