PMID: 9450338Feb 5, 1998Paper

The quiescent/colorless alleles of viviparous1 show that the conserved B3 domain of VP1 is not essential for ABA-regulated gene expression in the seed

The Plant Journal : for Cell and Molecular Biology
C. B. CarsonD R McCarty

Abstract

A series of vp1 alleles distinguish at least two classes of maturation-related genes that are regulated by the VP1 factor and abscisic acid (ABA). The intermediate vp1-c821708 and vp1-McW alleles have quiescent (non-viviparous) anthocyanin-deficient phenotypes while maintaining significant levels of maturation-specific gene expression in the developing embryo. However, expression of the C1 regulatory gene of the anthocyanin pathway is not detected in these mutants. Reduced steady-state levels of structurally altered VP1 proteins are detected in quiescent mutant embryos. The VP1-McW protein sequence lacks the highly conserved region encoded by exons 3-5 of the Vp1 gene. A sensitive RT-PCR assay was used to rule out significant amounts of intact transcripts in the vp1-McW mutant that could account for the quiescent phenotype. In transient expression assays, the VP1-McW protein and other mutants with a truncated B3 domain of VP1 retained a strong capacity to synergistically enhance ABA-regulation of the Em-GUS reporter gene; whereas transactivation of both Em-GUS and C1-sh-GUS genes in the absence of hormone was strongly inhibited. These results indicate that the largest conserved region in VP1 homologs (B3) is critical for gene a...Continue Reading

Citations

Dec 18, 2004·Plant Molecular Biology·Raúl CarrancoTimothy C Hall
Feb 27, 1999·Current Opinion in Plant Biology·U Wobus, H Weber
Nov 26, 1999·The Plant Journal : for Cell and Molecular Biology·T HoboT Hattori
Aug 8, 2001·The Plant Journal : for Cell and Molecular Biology·S NakamuraR R Finkelstein
Dec 12, 2001·The Plant Journal : for Cell and Molecular Biology·M SuzukiD R McCarty
May 29, 2008·Genes & Genetic Systems·Shigeko UtsugiMasahiko Maekawa
May 13, 1999·Proceedings of the National Academy of Sciences of the United States of America·T UlmasovT J Guilfoyle
Jul 18, 2002·Proceedings of the National Academy of Sciences of the United States of America·Rowan S McKibbinMichael J Holdsworth
Dec 28, 1999·Proceedings of the National Academy of Sciences of the United States of America·T HoboT Hattori
Dec 18, 2012·The Plant Journal : for Cell and Molecular Biology·Doron Shkolnik-InbarDudy Bar-Zvi
Nov 1, 2000·Cell·D R McCarty, J Chory
Sep 22, 2016·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Yingjun ZhangZhonghu He
Oct 27, 2004·The Journal of Biological Chemistry·Xiaolu ZouQingxi J Shen
Jan 1, 2002·The Arabidopsis Book·Ruth R Finkelstein, Christopher D Rock

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