The Rab4 effector Rabip4 plays a role in the endocytotic trafficking of Glut 4 in 3T3-L1 adipocytes

Journal of Cell Science
Muriel MariM Cormont

Abstract

Insulin regulates glucose uptake in the adipocytes by modulating Glut 4 localization, a traffic pathway involving the endocytic small GTPases Rab4, Rab5, and RabThe expression of the Rab4 effector Rabip4 leads to a 30% increase in glucose uptake and Glut 4 translocation in the presence of insulin, without modifications in the basal condition. This effect was not due to modifications of Glut 4 expression or insulin signaling, suggesting that Rabip4 controls Glut 4 trafficking. We present evidence that Rabip4 defines a subdomain of early endosomes and that Rabip4 is redistributed to the plasma membrane by insulin. Rabip4 is mostly absent from structures positive for early endosome antigen 1, Rab11 or transferrin receptors and from Glut 4 sequestration compartments. However, Rabip4 vesicles can be reached by internalized transferrin and Glut 4. Thus, Rabip4 probably defines an endocytic sorting platform for Glut 4 towards its sequestration pool. The expression of a form of Rabip4 unable to bind Rab4 does not modify basal and insulin-induced glucose transport. However, it induces an increase in the amount of Glut 4 at the plasma membrane and perturbs Glut 4 traffic from endosomes towards its sequestration compartments. These observ...Continue Reading

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Citations

May 24, 2012·Nature Reviews. Molecular Cell Biology·Dara Leto, Alan R Saltiel
Jun 11, 2010·Molecular Biology of the Cell·Hideaki YamamotoHye-Won Shin
Jan 4, 2008·Acta Physiologica·V KaddaiM Cormont
Feb 28, 2013·Journal of Cell Science·Sam E ReedJeremy M Tavaré
Sep 6, 2014·Biologie aujourd'hui·Franck CeppoJean-François Tanti
Jul 24, 2007·Current Opinion in Cell Biology·June Chunqiu Hou, Jeffrey E Pessin
Dec 15, 2010·Seminars in Cell & Developmental Biology·Shreya MitraGordon B Mills
Dec 1, 2015·Cellular Microbiology·Hiroki TakeuchiAtsuo Amano
Mar 23, 2013·International Journal of Molecular Sciences·Yasuko Kitagishi, Satoru Matsuda
Sep 27, 2006·The Journal of Biological Chemistry·Jelena VukmiricaMireille Cormont
Mar 5, 2016·The Biochemical Journal·Paul Duffield BrewerCynthia Corley Mastick

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