The radical site in chlamydial ribonucleotide reductase defines a new R2 subclass

Science
Martin HögbomPär Nordlund

Abstract

Ribonucleotide reductase (RNR) synthesizes the deoxyribonucleotides for DNA synthesis. The R2 protein of normal class I ribonucleotide reductases contains a diiron site that produces a stable tyrosyl free radical, essential for enzymatic activity. Structural and electron paramagnetic resonance studies of R2 from Chlamydia trachomatis reveal a protein lacking a tyrosyl radical site. Instead, the protein yields an iron-coupled radical upon reconstitution. The coordinating structure of the diiron site is similar to that of diiron oxidases/monoxygenases and supports a role for this radical in the RNR mechanism. The specific ligand pattern in the C. trachomatis R2 metal site characterizes a new group of R2 proteins that so far has been found in eight organisms, three of which are human pathogens.

References

Mar 1, 1991·Microbiological Reviews·J W Moulder
Aug 18, 1994·Nature·U Uhlin, H Eklund
Apr 1, 1996·The Journal of Infectious Diseases·R C BrunhamF A Plummer
Jan 1, 1997·Annual Review of Immunology·J MacMickingC Nathan
Apr 7, 1998·Infection and Immunity·J U IgietsemeH D Caldwell
Aug 26, 1998·Cellular and Molecular Life Sciences : CMLS·M Fontecave
Oct 6, 1998·Annual Review of Biochemistry·A Jordan, P Reichard
Sep 15, 1999·Cellular and Molecular Life Sciences : CMLS·O GuittetM Lepoivre
Sep 14, 2000·The Journal of Biological Chemistry·C RoshickG McClarty
Feb 14, 2002·Progress in Biophysics and Molecular Biology·H EklundP Nordlund
Mar 8, 2003·Proceedings of the National Academy of Sciences of the United States of America·Martin HögbomFriedhelm Lendzian
Apr 10, 2004·Science·Matthias HornMichael Wagner

❮ Previous
Next ❯

Citations

Feb 3, 2005·Nature Reviews. Immunology·Robert C Brunham, José Rey-Ladino
May 22, 2008·Proceedings of the National Academy of Sciences of the United States of America·Joseph P EmersonLawrence Que
Mar 27, 2009·Proceedings of the National Academy of Sciences of the United States of America·Charlotta S Andersson, Martin Högbom
Oct 9, 2013·Proceedings of the National Academy of Sciences of the United States of America·Julia J GrieseMartin Högbom
Aug 12, 2010·Molecular Plant-microbe Interactions : MPMI·Michiko E Taga, Graham C Walker
Sep 3, 2009·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Hideaki OgataWolfgang Lubitz
Oct 26, 2010·Journal of Bacteriology·Silvia KnabIlka Haferkamp
Jun 8, 2006·Annual Review of Biochemistry·Pär Nordlund, Peter Reichard
Dec 15, 2010·BMC Evolutionary Biology·Daniel LundinAnthony M Poole
Feb 9, 2013·BMC Evolutionary Biology·Bhakti DwivediMya Breitbart
Dec 16, 2010·Future Microbiology·Melanie CochraneKenneth W Beagley
Jun 17, 2006·Proceedings of the National Academy of Sciences of the United States of America·N VoevodskayaA Gräslund
Apr 5, 2011·Annual Review of Biochemistry·Joseph A Cotruvo, Joanne Stubbe
May 4, 2013·Annual Review of Plant Biology·Anthony L MooreKikukatsu Ito
Apr 29, 2014·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Julia J GrieseMartin Högbom
Feb 13, 2013·The Journal of Biological Chemistry·Kajsa G V SigfridssonMichael Haumann
Aug 4, 2012·Metallomics : Integrated Biometal Science·Julia J Griese, Martin Högbom
Dec 4, 2013·ACS Chemical Biology·Marta HammerstadK Kristoffer Andersson
Sep 4, 2007·Journal of Molecular Biology·Georg ZocherGeorg E Schulz
Nov 9, 2007·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Konstantinos D PapavasileiouVasilios S Melissas
Sep 2, 2009·Physiologia Plantarum·Mary S AlburyAnthony L Moore

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.