PMID: 8606055Jan 1, 1996Paper

The RAG cell line defines a new complementation group of MHC class II deficiency

Immunogenetics
A M LennonC Alcaïde-Loridan

Abstract

We previously described RAG, a mouse adenocarcinoma cell line, as deficient for the induction of major histocompatibility (MHC) class II antigens by IFN-gamma, but responding normally for MHC class I antigen stimulation and anti-viral protection. We had established that the fusion of RAG with various human cell lines restored the induction of MHC class II antigens, whenever the human chromosome 16 was present in somatic cell hybrids. Here we show that the RAG cell line does not exhibit any induction by IFN-gamma of DMA, DMB, and the invariant chain (Ii) mRNAs, and that the induction is restored in somatic cell hybrids containing human chromosome 16. In order to define the gene (designated F16) affected in the RAG cells, we performed a complementation analysis by fusing RAG with previously described human cell lines defective for MHC class II antigen expression (e.g., BLS cell lines), and which belong to five different complementation groups. Our data show that the resulting somatic cell hybrids present an inducible expression of mouse MHC class II antigens, Ii, DMA, and DMB. Therefore, the RAG cell line represents a yet undescribed cellular mutant affected in the expression of MHC class II antigens. In addition, we demonstrate ...Continue Reading

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Citations

Feb 20, 2004·Leukemia·T Prod'hommeC Alcaide-Loridan
Apr 30, 2002·International Immunology·Rodrigo NavesMaria Rosa Bono
Sep 7, 2002·The Journal of Biological Chemistry·Santasabuj DasPhilip N Tsichlis
Jun 22, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·V DeffrennesC Alcaïde-Loridan
Apr 21, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·A M Lennon-DuménilC Alcaïde-Loridan

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